2009年1月
Ca2+ induces macropinocytosis via F-actin depolymerization during growth cone collapse
MOLECULAR AND CELLULAR NEUROSCIENCE
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 40
- 号
- 1
- 開始ページ
- 27
- 終了ページ
- 38
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.mcn.2008.08.009
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
Growth cone collapse occurs in repulsive axon guidance and is accompanied by a reduction in the surface area of the plasma membrane of growth cones. However, the mechanism of this reduction is unclear. Here, we show that during growth cone collapse, caffeine-induced Ca2+ release from ryanodine-sensitive Ca2+ Stores triggers the formation of large vacuoles in growth cones by macropinocytosis, a clathrin-independent endocytosis for the massive retrieval of the cellular plasma membrane, and Subsequent retrograde membrane transport. We observed a significant correlation of the area of caffeine-induced macropinosomes with growth cone collapse. We also detected macropinocytosis induced by semaphorin 3A, a typical repulsive cue, and correlation between the area of semaphorin 3A-induced macropinocytic vacuoles and growth cone collapse. Moreover, jasplakinolide, an inhibitor of F-actin depolymerization, blocked caffeine-induced macropinocytosis. We propose that the coordinated regulation of actin cytoskeletal reorganization and macropinocytosis-mediated retrograde membrane trafficking may contribute to Ca2+-induced axon growth inhibition. (C) 2008 Elsevier Inc. All rights reserved.
- リンク情報
- ID情報
-
- DOI : 10.1016/j.mcn.2008.08.009
- ISSN : 1044-7431
- PubMed ID : 18848894
- Web of Science ID : WOS:000262422700003