2012年8月
Diverse lectin-binding specificity of four ZP3 glycoprotein isoforms with a discrete isoelectric point in chicken egg coat
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
- ,
- ,
- ,
- ,
- ,
- 巻
- 424
- 号
- 3
- 開始ページ
- 586
- 終了ページ
- 592
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bbrc.2012.06.157
- 出版者・発行元
- ACADEMIC PRESS INC ELSEVIER SCIENCE
The vertebrate egg coat corresponding to mammalian zona pellucida is a filamentous matrix composed of highly and heterogeneously glycosylated proteins designated ZP glycoproteins including ZP1 to 4, ZPD and ZPAX, and play important roles in species-specific egg-sperm interactions. Recent advance in structural biology of chicken ZP3 provided new insights into molecular mechanisms of the egg-coat function involving its carbohydrate moieties. In this study, chicken ZP3 was separated into four major and distinct isoforms with different pI in 2D-PAGE. To investigate the meanings of the ZP3 heterogeneity in egg-sperm interactions, we preliminary analyzed glycan diversity on the molecules by using lectin-staining assays. The four major ZP3 isoforms 4-7 (from acidic to basic) were recognized equally with PNA (Gal beta 1-3GaINAc), but the isoforms 5-7 were recognized dominantly with WGA ((beta-GlcNAc)n, clustered Sia), PHA-E (bi- and triantennary N-glycan containing Gal beta 1-4GlcNAc beta 1-2Man alpha 1 -6) and RCA I (terminal Gal beta 1-4GlcNAc), respectively. Despite such sugar chain diversity among the ZP3 isoforms, a partner in the egg coat, ZP1, showed specific binding to each isoform equally. Localization of ZP1 and ZP3 in the egg-coat matrix were also analyzed. Crown Copyright (C) 2012 Published by Elsevier Inc. All rights reserved.
- リンク情報
-
- DOI
- https://doi.org/10.1016/j.bbrc.2012.06.157
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22776203
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000307618800038&DestApp=WOS_CPL
- 共同研究・競争的資金等の研究課題
- 鳥類の卵・精子相互認識に関与する精子膜分子の同定および卵膜との相互作用機構の解明
- URL
- http://www.sciencedirect.com/science/article/pii/S0006291X12012703
- ID情報
-
- DOI : 10.1016/j.bbrc.2012.06.157
- ISSN : 0006-291X
- PubMed ID : 22776203
- Web of Science ID : WOS:000307618800038