2001年9月
Human glycosaminoglycan glucuronyltransferase I gene and a related processed pseudogene: genomic structure, chromosomal mapping and characterization
BIOCHEMICAL JOURNAL
- ,
- ,
- ,
- 巻
- 358
- 号
- 開始ページ
- 539
- 終了ページ
- 546
- 記述言語
- 英語
- 掲載種別
- DOI
- 10.1042/0264-6021:3580539
- 出版者・発行元
- PORTLAND PRESS
Here we describe the characterization of the human glycosaminoglycan glucuronyltransferase I gene (GlcAT-I) and a related pseudogene. The GlcAT-I gene was localized to human chromosome 11q12-q13 by in situ hybridization of metaphase chromosomes. GlcAT-I spanned 7 kb of human genomic DNA and was divided into five exons. Northern blot analysis showed that GlcAT-I exhibited ubiquitous but markedly different expressions in the human tissues examined. The GlcAT-I promoter was approx. 3-fold more active in a melanoma cell line than in a hepatoma cell line, providing evidence for the differential regulation of the gene's expression. Stepwise 5` deletions of the promoter identified a strong enhancer element between -303 and -153 by that included binding motifs for Ets, CREB (CAMP-response-element-binding protein) and STAT (signal transducers and activators of transcription). Screening of a human genomic library identified one additional distinct genomic clone containing an approx. 1.4 kb sequence region that shared an overall 95.3 % nucleotide identity with exons 1-5 of GlcAT-I However, a lack of intron sequences, as well as the presence of several nucleotide mutations, insertions and deletions that disrupted the potential GlcAT-I reading frame, suggested that the clone contained a processed pseudogene. The pseudogene was localized to chromosome 3. The human genome therefore contains two related GlcAT-I genes that are located on separate chromosomes.
- リンク情報
-
- DOI
- https://doi.org/10.1042/0264-6021:3580539
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/11535117
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000171258900002&DestApp=WOS_CPL
- URL
- https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=0035884414&origin=inward
- ID情報
-
- DOI : 10.1042/0264-6021:3580539
- ISSN : 0264-6021
- PubMed ID : 11535117
- SCOPUS ID : 0035884414
- Web of Science ID : WOS:000171258900002