2018年5月
Immunohistochemical localization of osteoblast activating peptide in the mouse kidney.
Acta histochemica
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- ,
- ,
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- 巻
- 120
- 号
- 4
- 開始ページ
- 323
- 終了ページ
- 328
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.acthis.2018.03.001
- 出版者・発行元
- ELSEVIER GMBH, URBAN & FISCHER VERLAG
Osteoblast activating peptide (OBAP) is a newly discovered peptide detected in the rat stomach, which has a major role in osteogenesis. Recently, we revealed its localization in the parietal cells of the rat stomach. There have been no data regarding OBAP expression in the kidney, despite its role in calcium reabsorption in renal tubules. The current study aimed to inspect the expression of OBAP in the kidney of twelve 10-week-old male C3H/HeNJc1 mice using immunohistochemistry, and immunoelectron microscopic localization. The immunohistochemical investigation revealed an OBAP positive reaction mainly in the medulla, which was stronger than the cortex of the kidney and was concentrated in the distal convoluted tubules (DCT), connecting tubules (CT), and the thick limbs of the loop of Henle (HL). Moreover, we clarified that the OBAP was co-distributed with ghrelin and calbindin (markers of the DCT). Interestingly, immunoelectron microscopy demonstrated that OBAP was concentrated in the mitochondrial inner membrane of the DCT and CT. Based on these results, it was concluded that the mitochondria of the DCT, CT, and HL of the mice kidney generate OBAP. Furthermore, our results suggest that OBAP might have a role in the regulation of calcium reabsorption by the renal tubule; however, further investigations are required to clarify this potential role.
- リンク情報
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- DOI
- https://doi.org/10.1016/j.acthis.2018.03.001
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/29534834
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000436055000003&DestApp=WOS_CPL
- URL
- http://www.scopus.com/inward/record.url?eid=2-s2.0-85043334387&partnerID=MN8TOARS
- Scopus Citedby
- https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=85043334387&origin=inward
- ID情報
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- DOI : 10.1016/j.acthis.2018.03.001
- ISSN : 0065-1281
- eISSN : 1618-0372
- ORCIDのPut Code : 98814437
- PubMed ID : 29534834
- SCOPUS ID : 85043334387
- Web of Science ID : WOS:000436055000003