論文

査読有り
2011年2月

Transcriptome Analysis of Respiration-Responsive Genes in Chlamydomonas reinhardtii: Mitochondrial Retrograde Signaling Coordinates the Genes for Cell Proliferation with Energy-Producing Metabolism

PLANT AND CELL PHYSIOLOGY
  • Mitsuhiro Matsuo
  • ,
  • Rei Hachisu
  • ,
  • Satoshi Tabata
  • ,
  • Hideya Fukuzawa
  • ,
  • Junichi Obokata

52
2
開始ページ
333
終了ページ
343
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcq192
出版者・発行元
OXFORD UNIV PRESS

Plant organelles are not only the recipients of signals from the nucleus, but also elicit signals to regulate nuclear genes; the latter process is called retrograde regulation. We previously reported a novel mitochondrial retrograde regulation in Chlamydomonas reinhardtii; nuclear photosynthesis genes are regulated in response to mitochondrial respiratory electron transport (RET). However, the physiological roles of this retrograde regulation are not yet fully understood. In this study, we performed a genome-wide transcriptome analysis of this alga to reveal what kinds of genes are responsive to this RET signal, using Chlamydomonas macroarrays containing 10,368 expressed sequence tag clones. From the analysis, we identified 147 inducible and 35 repressive genes based on a couple of criteria: induction/repression by activated respiration caused by exogenously added acetate, and the cancellations of these responses by treatment with antimycin A, an inhibitor of RET. Interestingly, genes for respiration, photosynthesis, glycolysis/gluconeogenesis, protein biosynthesis, cell wall biogenesis and flagella were significantly induced by RET-derived signals. From these findings, we discuss the physiological role of mitochondrial retrograde signaling in this unicellular alga, in terms of the coordination of cell proliferation with energy-producing metabolism.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1093/pcp/pcq192
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/21149298
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000287254000014&DestApp=WOS_CPL

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