論文

査読有り
2008年1月

Two RNA editing sites with cis-acting elements of moderate sequence identity are recognized by an identical site-recognition protein in tobacco chloroplasts

NUCLEIC ACIDS RESEARCH
  • Yusuke Kobayashi
  • ,
  • Mitsuhiro Matsuo
  • ,
  • Koji Sakamoto
  • ,
  • Tatsuya Wakasugi
  • ,
  • Kyoji Yamada
  • ,
  • Junichi Obokata

36
1
開始ページ
311
終了ページ
318
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/nar/gkm1026
出版者・発行元
OXFORD UNIV PRESS

The chloroplast genome of higher plants contains 2040 C-to-U RNA editing sites, whose number and locations are diversified among plant species. Biochemical analyses using in vitro RNA editing systems with chloroplast extracts have suggested that there is one-to-one recognition between proteinous site recognition factors and their respective RNA editing sites, but their rigidness and generality are still unsettled. In this study, we addressed this question with the aid of an in vitro RNA editing system from tobacco chloroplast extracts and with UV-crosslinking experiments. We found that the ndhB-9 and ndhF-1 editing sites of tobacco chloroplast transcripts are both bound by the site-specific trans-acting factors of 95 kDa. Cross-competition experiments between ndhB-9 and ndhF-1 RNAs demonstrated that the 95 kDa proteins specifically binding to the ndhB-9 and ndhF-1 sites are the identical protein. The binding regions of the 95 kDa protein on the ndhB-9 and ndhF-1 transcripts showed 60% identity in nucleotide sequence. This is the first biochemical demonstration that a site recognition factor of chloroplast RNA editing recognizes plural sites. On the basis of this finding, we discuss how plant organellar RNA editing sites have diverged during evolution.

Web of Science ® 被引用回数 : 29

リンク情報
DOI
https://doi.org/10.1093/nar/gkm1026
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/18032432
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000252545100037&DestApp=WOS_CPL

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