論文

査読有り
2005年3月

The rice nuclear genome continuously integrates, shuffles, and eliminates the chloroplast genome to cause chloroplast-nuclear DNA flux

PLANT CELL
  • M Matsuo
  • ,
  • Y Ito
  • ,
  • R Yamauchi
  • ,
  • J Obokata

17
3
開始ページ
665
終了ページ
675
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1105/tpc.104.027706
出版者・発行元
AMER SOC PLANT BIOLOGISTS

Plastid DNA fragments are often found in the plant nuclear genome, and DNA transfer from plastids to the nucleus is ongoing. However, successful gene transfer is rare. What happens to compensate for this? To address this question, we analyzed nuclear-localized plastid DNA (nupDNA) fragments throughout the rice (Oryza sativa ssp japonica) genome, with respect to their age, size, structure, and integration sites on chromosomes. The divergence of nupDNA sequences from the sequence of the present plastid genome strongly suggests that plastid DNA has been transferred repeatedly to the nucleus in rice. Age distribution profiles of the nupDNA population, together with the size and structural characteristics of each fragment, revealed that once plastid DNAs are integrated into the nuclear genome, they are rapidly fragmented and vigorously shuffled, and surprisingly, 80% of them are eliminated from the nuclear genome within a million years. Large nupDNA fragments preferentially localize to the pericentromeric region of the chromosomes, where integration and elimination frequencies are markedly higher. These data indicate that the plant nuclear genome is in equilibrium between frequent integration and rapid elimination of the chloroplast genome and that the pericentromeric regions play a significant role in facilitating the chloroplast-nuclear DNA flux.

Web of Science ® 被引用回数 : 104

リンク情報
DOI
https://doi.org/10.1105/tpc.104.027706
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15705954
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000227685600003&DestApp=WOS_CPL

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