論文

査読有り
2002年10月

Dual roles of photosynthetic electron transport in photosystem I biogenesis: Light induction of mRNAs and chromatic regulation at post-mRNA level

PLANT AND CELL PHYSIOLOGY
  • M Matsuo
  • ,
  • J Obokata

43
10
開始ページ
1189
終了ページ
1197
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcf146
出版者・発行元
OXFORD UNIV PRESS

Light regulation of photosystem I (PSI) biogenesis was studied in a unicellular green alga, Chlamydomonas reinhardtii. When Chlamydomonas cells were transferred from darkness to the light, mRNAs for both nuclear- and chloroplast-en coded PSI subunits were induced in concert. This light induction was inhibited by photosynthetic electron transport (PET) inhibitors, 3-(3,4 dichlorophenyl)1,1-dimethylurea and 2,5-dibromo-3-methyl-6 isopropyl-p-benzoquinone, but not by an uncoupler, carbonyl cyanide m-chlorophenylhydrazone. This indicated that PET plays a pivotal role in the light induction of PSI subunit mRNAs, but that photophosphorylation is not necessary. When we irradiated the Chlamydomonas cells with PSI-light (695 nm) or PSII-light (644 nm), which makes the plastoquinone pool oxidative and reductive, respectively, PSI-light caused the accumulation of PSI proteins more abundantly than did PSII-light. However, there was no difference for the PSI subunit mRNA levels between these light sources. From these results, we conclude that PET plays dual roles in the regulation of PSI biogenesis in Chlamydomonas: when cells are illuminated, PET first induces the PSI subunit mPNAs irrespective of the redox state of the intersystem electron carriers, and then their redox state fine-tunes PSI biogenesis at translational and/or post-translational steps to fulfil the chromatic adaptation.

Web of Science ® 被引用回数 : 11

リンク情報
DOI
https://doi.org/10.1093/pcp/pcf146
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/12407199
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000178848100014&DestApp=WOS_CPL

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