論文

査読有り 国際誌
2016年

Intraperitoneal Mesenchymal Cells Promote the Development of Peritoneal Metastasis Partly by Supporting Long Migration of Disseminated Tumor Cells.

PloS one
  • Joji Kitayama
  • ,
  • Hironori Yamaguchi
  • ,
  • Hironori Ishigami
  • ,
  • Keisuke Matsuzaki
  • ,
  • Naohiro Sata

11
5
開始ページ
e0154542
終了ページ
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1371/journal.pone.0154542
出版者・発行元
PUBLIC LIBRARY SCIENCE

The human peritoneal cavity contains a small number of free cells of mesenchymal cell lineage. Intraperitoneal mesenchymal cells (PMC) play supportive roles in metastasis formation on the peritoneum. In this study, we found that PMC, when co-cultuerd with human gastric cancer cells, MKN45, enhanced the proliferation of MKN45 when cultured at low, but not high, cellular density. Also, PMC suppressed apoptotic cell death of MKN45 only under low density culture conditions. Time-lapse videoanalysis clearly demonstrated that PMC randomly migrated more vigorously than did MKN45, and strongly enhanced the migration behavior of co-cultured MKN45. In fact, the majority of MKN45 migrated together in direct physical contact with PMC, and the sum of migration lengths from original position of co-cultured MKN45 for 48 hours was approximately 10 times longer than that of MKN45 cultured alone. Our data suggest that enhanced migration can increase the chance of direct contact or positional proximity among sparcely distributed MKN45, which may bring survival advantages to tumor cells. This may be one of the important mechanisms of peritoneal metastasis, since only a small number of tumor cells are considered to be disseminated in the early step of metastasis formation on the peritoneum.

リンク情報
DOI
https://doi.org/10.1371/journal.pone.0154542
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/27136922
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4854412
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000375675700040&DestApp=WOS_CPL
ID情報
  • DOI : 10.1371/journal.pone.0154542
  • ISSN : 1932-6203
  • PubMed ID : 27136922
  • PubMed Central 記事ID : PMC4854412
  • Web of Science ID : WOS:000375675700040

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