2018年4月1日
Glycine is a predictor for a suppressive effect of fucoxanthinol on colonosphere formation under hypoxia
Anticancer Research
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- 巻
- 38
- 号
- 4
- 開始ページ
- 2169
- 終了ページ
- 2179
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.21873/anticanres.12458
- 出版者・発行元
- International Institute of Anticancer Research
Background/Aim: Fucoxanthinol (FxOH), a metabolite of fucoxanthin, is known to inhibit tumorigenicity of human colorectal cancer stem cells (CCSCs) and their sphere formation. Hypoxic conditions and hypoxia-inducible factors (HIFs) are essential to maintain the stemness of CCSCs. We investigated effects of FxOH on sphere formation, intercellular energy metabolites in colonospheres formed from human colorectal HT-29 cells under hypoxic conditions. Results: FxOH at 50 μM suppressed HIF1α expression and activation of integrin, mitogen-activated protein kinase (MAPK), Wingless/integrated (WNT) and signal transducer and activator of transcription (STAT) signals. Moreover, expression of epithelial–mesenchymal transition- and apoptosis-related proteins in the colonospheres was lowered by FxOH. The level of glycine was reduced in hypoxic colonospheres under FxOH treatment. Conclusion: FxOH attenuated the sphere formation of hypoxic colonospheres, in part, by suppressing HIF1α expression. Glycine could be a potential predictor for the activity of agents that inhibit sphere formation by hypoxic colonospheres.
- リンク情報
- ID情報
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- DOI : 10.21873/anticanres.12458
- ISSN : 1791-7530
- ISSN : 0250-7005
- PubMed ID : 29599336
- SCOPUS ID : 85045181576