2010年6月
Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation
DEVELOPMENTAL NEUROBIOLOGY
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- ,
- ,
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- 巻
- 70
- 号
- 7
- 開始ページ
- 523
- 終了ページ
- 537
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/dneu.20794
- 出版者・発行元
- WILEY-BLACKWELL
After the initial primary projection, axons undergo various structural and functional changes to establish mature neural circuits. The changes in protein expression associated with this maturation were investigated in lateral olfactory tract axons using two-dimensional gel electrophoresis. The most prominent group upregulated during the period consisted of calcium-dependent membrane-binding proteins including VILIP1, neurocalcin 8, copine 6, and annexin A6 from three structurally different families. During maturation of primary cultured neurons, annexin A6 gradually became concentrated on the axon initial segment, and its overexpression significantly enhanced axon branching. On the other hand, overexpression of VILIP1 and neurocalcin 8 reduced axon outgrowth and branching. The second group upregulated during axon maturation comprised tubulin- and microtubule-binding proteins including CRMP2, guanine deaminase, MAP1B, and tibronectin type3 SPRY domain-containing protein. Because the maturation of lateral olfactory axons involves massive extension of secondary collateral branches, the augmentation of these proteins during these stages may underlie the drastic restructuring of the axon cytoskeleton. (C) 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 523-537, 2010
- リンク情報
- ID情報
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- DOI : 10.1002/dneu.20794
- ISSN : 1932-8451
- PubMed ID : 20225247
- Web of Science ID : WOS:000278355600005