論文

査読有り
2010年6月

Proteomics Analysis of the Temporal Changes in Axonal Proteins during Maturation

DEVELOPMENTAL NEUROBIOLOGY
  • Hitoshi Yamatani
  • ,
  • Takahiko Kawasaki
  • ,
  • Sakura Mita
  • ,
  • Naoyuki Inagaki
  • ,
  • Tatsumi Hirata

70
7
開始ページ
523
終了ページ
537
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/dneu.20794
出版者・発行元
WILEY-BLACKWELL

After the initial primary projection, axons undergo various structural and functional changes to establish mature neural circuits. The changes in protein expression associated with this maturation were investigated in lateral olfactory tract axons using two-dimensional gel electrophoresis. The most prominent group upregulated during the period consisted of calcium-dependent membrane-binding proteins including VILIP1, neurocalcin 8, copine 6, and annexin A6 from three structurally different families. During maturation of primary cultured neurons, annexin A6 gradually became concentrated on the axon initial segment, and its overexpression significantly enhanced axon branching. On the other hand, overexpression of VILIP1 and neurocalcin 8 reduced axon outgrowth and branching. The second group upregulated during axon maturation comprised tubulin- and microtubule-binding proteins including CRMP2, guanine deaminase, MAP1B, and tibronectin type3 SPRY domain-containing protein. Because the maturation of lateral olfactory axons involves massive extension of secondary collateral branches, the augmentation of these proteins during these stages may underlie the drastic restructuring of the axon cytoskeleton. (C) 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 523-537, 2010

リンク情報
DOI
https://doi.org/10.1002/dneu.20794
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/20225247
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000278355600005&DestApp=WOS_CPL
ID情報
  • DOI : 10.1002/dneu.20794
  • ISSN : 1932-8451
  • PubMed ID : 20225247
  • Web of Science ID : WOS:000278355600005

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