2006年4月
Identification of enzyme responsible for erythritol utilization and reaction product in yeast Lipomyces starkeyi
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
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- 巻
- 101
- 号
- 4
- 開始ページ
- 303
- 終了ページ
- 308
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1263/jbb.101.303
- 出版者・発行元
- SOC BIOSCIENCE BIOENGINEERING JAPAN
We have identified the enzyme responsible for erythritol utilization and its reaction product in the yeast Lipomyces starkeyi CBS 1807. The enzyme, a polyol dehydrogenase requiring NAD(+) as a coenzyme, was induced by erythritol in this yeast. We confirmed that the enzyme product was L-erythrulose by MS, NMR, and polarimeter analyses, meaning that we clarified the first step of erythritol utilization in yeasts for the first time. In the case of the oxidative reaction, D-threitol, (2R,3R)-2,3-butanediol, and erythritol were much better substrates than 21 other polyols tested. These three substrates are tetroses and have an R configuration at C-3, and whose third carbon results in easiest oxidation in this enzyme. The research of the substrate specificity in the reductive reaction demonstrated that L-erythrulose and dihydroxyacetone were better substrates, that D-acetoin was inactive and L-erythrose (aldose) was slightly active.
- リンク情報
- ID情報
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- DOI : 10.1263/jbb.101.303
- ISSN : 1389-1723
- Web of Science ID : WOS:000238324700003