論文

査読有り
1999年8月

Endoderm differentiation and inductive effect of activin-treated ectoderm in Xenopus

DEVELOPMENT GROWTH & DIFFERENTIATION
  • H Ninomiya
  • ,
  • S Takahashi
  • ,
  • K Tanegashima
  • ,
  • C Yokota
  • ,
  • M Asashima

41
4
開始ページ
391
終了ページ
400
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1046/j.1440-169X.1999.00449.x
出版者・発行元
BLACKWELL SCIENCE ASIA

When presumptive ectoderm is treated with high concentrations of activin A, it mainly differentiates into axial mesoderm (notochord, muscle) in Xenopus and into yolk-rich endodermal cells in newt (Cynops pyrrhogaster). Xenopus ectoderm consists of multiple layers, different from the single layer of Cynops ectoderm. This multilayer structure of Xenopus ectoderm may prevent complete treatment of activin A and subsequent whole differentiation into endoderm. In the present study, therefore, Xenopus ectoderm was separated into an outer layer and an inner layer, which were individually treated with a high concentration of activin A (100 ng/mL). Then the differentiation and inductive activity of these ectodermal cells were examined in explantation and transplantation experiments. In isolation culture, ectoderm treated with activin A formed endoderm. Ectodermal and mesodermal tissues were seldom found in these explants. The activin-treated ectoderm induced axial mesoderm and neural tissues, and differentiated into endoderm when it was sandwiched between two sheets of ectoderm or was transplanted into the ventral marginal zone of other blastulae. These findings suggest that Xenopus ectoderm treated with a high concentration of activin A forms endoderm and mimics the properties of the organizer as in Cynops.

Web of Science ® 被引用回数 : 28

リンク情報
DOI
https://doi.org/10.1046/j.1440-169X.1999.00449.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10466926
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000082333600003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1046/j.1440-169X.1999.00449.x
  • ISSN : 0012-1592
  • PubMed ID : 10466926
  • Web of Science ID : WOS:000082333600003

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