論文

査読有り
2020年2月

Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide

SENSORS AND ACTUATORS B-CHEMICAL
  • Tasrina Munmun
  • ,
  • Arif Md. Rashedul Kabir
  • ,
  • Kazuki Sada
  • ,
  • Akira Kakugo

304
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.snb.2019.127231
出版者・発行元
ELSEVIER SCIENCE SA

Nanoscale transportation in engineered environments is critical towards designing efficient and smart hybrid bio-nanodevices. Biomolecular motors, the smallest natural machines, are promising as actuators as well as sensors in hybrid nanodevices and hold enormous potentials in nanoscale transportation. Highly specific regulation of the activity of biomolecular motors is the key to control such integrated nanodevices. We present a simple method to regulate the activity of a biomolecular motor system, microtubule (MT)-kinesin by using a natural osmolyte trimethylamine-N-oxide (TMAO). Motility of kinesin-driven MTs in an in vitro gliding assay is regulated over a broad spectrum by using TMAO in a concentration dependent manner. The regulation of MT motility is rapid, reversible and repeatable over multiple cycles. Interestingly, the motility of MTs can be completely turned off using TMAO of a relatively high concentration. The halted motility of MTs is fully restored upon elimination of TMAO. Repeated cycles of TMAO addition and removal enable cyclical inhibition and restoration of the motility of MTs. These results demonstrate an ability to control nanoscale motion of a biomolecular motor in an artificial environment. This work facilitates further tunability over functions of biomolecular motors, which in turn will foster their nanotechnological applications, such as in nano-transportation.

リンク情報
DOI
https://doi.org/10.1016/j.snb.2019.127231
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000500702500036&DestApp=WOS_CPL
ID情報
  • DOI : 10.1016/j.snb.2019.127231
  • eISSN : 0925-4005
  • Web of Science ID : WOS:000500702500036

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