論文

査読有り 国際誌
2019年

Monitoring Age-Related Changes in the Lactate/Pyruvate Ratio Using a Colorimetric Assay in a C. elegans Model of Increased Life Span.

Methods in molecular biology (Clifton, N.J.)
  • Sumino Yanase
  • ,
  • Kayo Yasuda
  • ,
  • Naoki Ishii

1916
開始ページ
123
終了ページ
132
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/978-1-4939-8994-2_12

In the nematode Caenorhabditis elegans (C. elegans), monitoring the lactate/pyruvate ratio in cells helps to detect imbalances in age-related energy metabolism. Here, we describe a modified small-scale extraction in C. elegans and measurement of lactate and pyruvate concentrations using colorimetric assay kits. During sample extraction, protein precipitation is the most critical step for precise determination of intracellular metabolites in C. elegans. Moreover, improved sensitivity and accuracy of colorimetric assay kits contributed to measurements of metabolites in samples derived from small-scale extraction. Using these protocols, we recently detected a metabolic alteration that occurs during aging by the monitoring the lactate/pyruvate ratio in a long-lived mutant of the mammalian tumor suppressor p53 ortholog CEP-1 in C. elegans.

リンク情報
DOI
https://doi.org/10.1007/978-1-4939-8994-2_12
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/30535690

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