2016年12月
Determination of genotoxic potential by comparison of structurally related azo dyes using DNA repair-deficient DT40 mutant panels
CHEMOSPHERE
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- 巻
- 164
- 号
- 開始ページ
- 106
- 終了ページ
- 112
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.chemosphere.2016.08.092
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
Azo dyes, including Sudan I, Orange II and Orange G, are industrial dyes that are assumed to have genotoxic potential. However, neither the type of DNA damage induced nor the structural features responsible for toxicity have been determined. We used a panel of DNA-repair-pathway-deficient mutants generated from chicken DT40 cells to evaluate the ability of these azo dyes to induce DNA damage and to identify the type of DNA damage induced. We compared the structurally related azo dyes Sudan I, Orange II and Orange G to identify the structural features responsible for genotoxicity. Compared with wild type cells, the double-strand break repair defective RAD54(-/-)/KU70(-/-) cells were significantly more sensitive to Sudan I, but not to Orange II or Orange G. The quantum-chemical calculations revealed that Sudan I, but not Orange II or Orange G, has a complete planar aromatic ring structure. These suggest that the planar feature of Sudan I is critical to the inducing of double-strand breaks. In summary, we used a DNA-repair mutant panel in combination with quantum-chemical calculations to provide a clue to the chemical structure responsible for genotoxicity. (C) 2016 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.chemosphere.2016.08.092
- ISSN : 0045-6535
- eISSN : 1879-1298
- Web of Science ID : WOS:000385318200014