論文

査読有り 国際誌
2021年7月9日

Enhancement of the Catalytic Activity of Hammerhead Ribozymes by Organic Cations.

Chembiochem : a European journal of chemical biology
  • Shu-Ichi Nakano
  • ,
  • Hirofumi Yamashita
  • ,
  • Naoki Sugimoto

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1002/cbic.202100280

Catalytic turnover is important for the application of ribozymes to biotechnology. However, the turnover is often impaired because of the intrinsic high stability of base pairs with cleaved RNA products. Here, organic cations were used as additives to improve the catalytic performance of hammerhead ribozyme constructs that exhibit different kinetic behaviors. Kinetic analysis of substrate cleavage demonstrated that bulky cations, specifically tetra-substituted ammonium ions containing pentyl groups or a benzyl group, have the ability to greatly increase the turnover rate of the ribozymes. Thermal stability analysis of RNA structures revealed that the bulky cations promote the dissociation of cleaved products and refolding of incorrectly folded structures with small disruption of the catalytic structure. The use of bulky cations is a convenient method for enhancing the catalytic activity of hammerhead ribozymes, and the approach may be useful for advancing ribozyme technologies.

リンク情報
DOI
https://doi.org/10.1002/cbic.202100280
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/34240789
ID情報
  • DOI : 10.1002/cbic.202100280
  • PubMed ID : 34240789

エクスポート
BibTeX RIS