Coniferous trees belonging to the family Cupressaceae are distributed worldwide. Several species of these conifers possess high economic value and have been a source of important forest products and raw materials for the forest industry. Somatic embryogenesis is a potential tool for clonal propagation of superior lineages within these conifers and for molecular breeding by genetic transformation. In this chapter, the technologies developed to date for somatic embryogenesis in trees of the Cupressaceae family are introduced, and various factors affecting somatic embryogenesis are discussed. In addition, methods for improving the efficiency of stable transformation via somatic embryogenesis are described.