Kotaro KONNO

J-GLOBAL         Last updated: Nov 27, 2018 at 20:05
Kotaro KONNO
National Agriculture and Food Research Organization
Research funding number

Research Areas


Academic & Professional Experience

Apr 2016
Institute of Agrobiological Scinences, National Agriculture and Food Research Institute (NARO)
Apr 2001
Mar 2016
National Institute of Agrobiological Scinences
Apr 1994
Mar 2001
National Institute of Sericultural and Entomological Science

Awards & Honors

Jan 2000
Discoveries on chemical components involved in plant defense agaisnt insect herbivory, Tsukuba Encouragement Prize (Young Scientist Category), The Science and Technology Promotion Foundation of Ibaraki


Unique modes of action of anti-inect defense proteins in plant latex: Plant-insect interaction madiated by latex of mulbery, papaya, and fig trees.
Kotaro KONNO
The Nature & Insects   53(11) 34-39   Oct 2018   [Invited]
Abnormal swelling of the peritrophic membrane in Eri silkworm gut caused by MLX56 family defense proteins with chitin-binding and extensin domains.
Kotaro KONNO
Phytochemistry   147 211-219   Mar 2018   [Refereed]
Kotaro Konno
Ecological Monographs   86 190-214   May 2016
© 2016 by the Ecological Society of America.Terrestrial ecosystems are generally green with vegetation and only a small part (<10%) of the total plant matter is consumed by herbivores annually, which means herbivore density is consistently low and...
Kotaro Konno, Takashi A. Inoue, Masatoshi Nakamura
PLoS ONE   9    Mar 2014
Raphides, needle-shaped calcium oxalate crystals in tissues of many plants, have been thought to play defensive roles against herbivores without detailed bioassays for their defensive roles and modes of function using purified raphides. In order t...
Eiji Ota, Wataru Tsuchiya, Toshimasa Yamazaki, Masatoshi Nakamura, Chikara Hirayama, Kotaro Konno
Phytochemistry   89 15-25   May 2013
Latex and other exudates in plants contain various proteins that are thought to play important defensive roles against herbivorous insects and pathogens. Herein, the defensive effects of phloem exudates against the Eri silkworm, Samia ricini (Satu...
Kei Kawazu, Kei Kawazu, Naoya Wasano, Naoya Wasano, Kotaro Konno, Yuko Ohashi, Atsushi Mochizuki, Ichiro Mitsuhara
Plant Biotechnology   29 495-499   Dec 2012
A high-throughput system for the evaluation of anti-herbivory genes in plant is proposed, in which genes are transiently expressed in a small area of leaf via Agrobacterium-infiltration, and the results can be obtained within 1 week. Transient exp...
Khondkar Ehteshamul Kabir, Daizo Koga, Kotaro Konno, Takuma Takanashi
Journal of Asia-Pacific Entomology   15 397-400   Sep 2012
This study investigated the potential of a fluorescein-5 isothiocyanate conjugated-chitin-binding domain (FITC-CBD) probe to detect chitinous materials in the peritrophic membrane (PM) structure of the Japanese pine sawyer beetle, Monochamus alter...
Kotaro Konno
Phytochemistry   72 1510-1530   Sep 2011
Plant latex and other exudates are saps that are exuded from the points of plant damage caused either mechanically or by insect herbivory. Although many (ca. 10%) of plant species exude latex or exudates, and although the defensive roles of plant ...
Kotaro Konno, Chikara Hirayama, Hiroe Yasui, Sachiko Okada, Masahiro Sugimura, Fumiko Yukuhiro, Yasumori Tamura, Makoto Hattori, Hiroshi Shinbo, Masatoshi Nakamura
Journal of Chemical Ecology   36 983-991   Sep 2010
The privet tree, Ligustrum obtusifolium (Oleaceae), defends its leaves against insects with a strong lysine-decreasing activity that make proteins non-nutritive. This is caused by oleuropein, an iridoid glycoside. We previously found that some pri...
Hisakazu Yamane, Kotaro Konno, Maurice Sabelis, Junji Takabayashi, Takeshi Sassa, Hideaki Oikawa
Comprehensive Natural Products II: Chemistry and Biology   4 339-385   Mar 2010
Kotaro Konno, Chikara Hirayama, Hiroshi Shinbo, Masatoshi Nakamura
Applied Entomology and Zoology   44 595-601   Dec 2009
The leaves of the privet tree, Ligustrum obtusifolium (Oleaceae), retain a strong lysine-decreasing activity caused by enzymatically-activated oleuropein, an iridoid glycoside. Protein treated with this activity become innutritive to insects becau...
Anurag A. Agrawal, Kotaro Konno
Annual Review of Ecology, Evolution, and Systematics   40 311-331   Dec 2009
Latex is a sticky emulsion that exudes upon damage from specialized canals in about 10% of flowering plant species. Latex has no known primary metabolic function and has been strongly implicated in defense against herbivorous insects. Here we revi...
Naoya Wasano, Kotaro Konno, Masatoshi Nakamura, Chikara Hirayama, Makoto Hattori, Ken Tateishi
Phytochemistry   70 880-888   May 2009
The mulberry (Morus spp.)-silkworm (Bombyx mori) relationship has been a well-known plant-herbivore interaction for thousands of years. Recently, we found that mulberry leaves defend against insect herbivory by latex ingredients. Here we report th...
植物防疫   62(5) 265-271   May 2008
Chikara Hirayama, Hiroshi Ono, Yasumori Tamura, Kotaro Konno, Masatoshi Nakamura
Phytochemistry   69 1141-1149   Mar 2008
The cocoons of some races of the silkworm, Bombyx mori, have been shown to contain 5-O-glucosylated flavonoids, which do not occur naturally in the leaves of their host plant, mulberry (Morus alba). Thus, dietary flavonoids could be biotransformed...
Chikara Hirayama, Kotaro Konno, Naoya Wasano, Masatoshi Nakamura
Insect Biochemistry and Molecular Biology   37 1348-1358   Dec 2007
Mulberry leaves (Morus spp.) exude latex rich in sugar-mimic alkaloids, 1,4-dideoxy-1,4-imino-d-arabinitol (d-AB1) and 1-deoxynojirimycin (DNJ), as a defense against herbivorous insects. Sugar-mimic alkaloids are inhibitors of sugar-metabolizing e...
ブレインテクノニュース   (115) 19-24   May 2006
今野浩太郎, 小野祐嗣, 中村匡利, 和佐野直也, 立石剣, 平山力, 田村泰盛, 服部誠, 小山朗夫, 河野勝行
農業生物資源研究所主要な研究成果   2005 10-11   May 2006
Kotaro Konno, Hiroshi Ono, Masatoshi Nakamura, Ken Tateishi, Chikara Hirayama, Yasumori Tamura, Makoto Hattori, Akio Koyama, Katsuyuki Kohno, Katsuyuki Kohno
Proceedings of the National Academy of Sciences of the United States of America   103 1337-1341   Jan 2006
Since ancient times, mulberry leaves (Morus spp.) have been used to rear the silkworm Bombyx mori. Because the silkworm grows well on mulberry leaves, the toxicities and defensive activities of these leaves against herbivorous insects have been ov...
Takayoshi Koike, Hiroyuki Tobita, Takanori Shibata, Sawako Matsuki, Sawako Matsuki, Kotaro Konno, Mitsutoshi Kitao, Naoko Yamashita, Yutaka Maruyama
Population Ecology   48 23-29   Jan 2006
Mixed broad-leaved forests are abundantly populated by several kinds of herbivore species in northern Japan. The life of herbivores depends strongly on the quality of food leaves. Leaf quality is changing with increasing atmospheric CO2 and nitrog...
Makoto Hattori, Makoto Hattori, Hirosato Konishi, Yasumori Tamura, Yasumori Tamura, Kotaro Konno, Kazushige Sogawa
Journal of Insect Physiology   51 1359-1365   Dec 2005
The activity and composition of leafhopper saliva are important in interactions with the host rice plant, and it may play a physiological role in detoxifying toxic plant substances or ingesting sap. We have characterized diphenoloxidase in the sal...
Yasumori Tamura, Makoto Hattori, Kotaro Konno, Hiroshi Honda, Yoshiaki Kono
Japanese Journal of Applied Entomology and Zoology   48 191-199   Nov 2004
The leaf beetle Ophraella communa is an oligophagous insect that infests several asteraceous plants, especially ragweed Ambrosia artemisiifolia, in fields of Japan. The feeding responses of O. communa to methanolic extracts of 11 genera and 17 spe...
今野浩太郎, 平山力, 中村匡利, 立石剣, 田村泰盛, 服部誠, 河野勝行
農業生物資源研究所主要な研究成果   2003 16-17   May 2004
Yasumori Tamura, Makoto Hattori, Kotaro Konno, Yoshiaki Kono, Hiroshi Honda, Hiroshi Ono, Mitsuru Yoshida
Chemoecology   14 113-118   Apr 2004
The leaf beetle Ophraella communa infests almost exclusively Ambrosia artemisiifolia in the fields of Japan, even though it normally feeds on several Asteraceous plants. A filter paper bioassay showed that the feeding of O. communa is strongly sti...
今野浩太郎, 武石勝, 百瀬清一, 高木久雄, 石橋晃
畜産の研究   58(4) 483-488   Apr 2004
Kotaro Konno, Chikara Hirayama, Masatoshi Nakamura, Ken Tateishi, Yasumori Tamura, Makoto Hattori, Katsuyuki Kohno
Plant Journal   37 370-378   Feb 2004
Many plants contain latex that exudes when leaves are damaged, and a number of proteins and enzymes have been found in it. The roles of those latex proteins and enzymes are as yet poorly understood. We found that papain, a cysteine protease in lat...
Kotaro Konno, Sachiko Okada, Chikara Hirayama
Journal of Insect Physiology   47 1451-1457   Dec 2001
The larva of the privet moth, Brahmaea wallichii (Brahmaeidae) is a specialist feeder of the privet tree, Ligustrum obtusifolium (Oleaceae). A very high concentration (50 mM or 0.4%) of free glycine, found in the digestive juice of the larvae, wor...
野蚕   (44) 3-5   Nov 2001
Kotaro Konno, Chikara Hirayama, Hiroe Yasui, Masatoshi Nakamura
Proceedings of the National Academy of Sciences of the United States of America   96 9159-9164   Aug 1999
Leaves of the privet tree, Ligustrum obtusifolium, contain a large amount of oleuropein, a phenolic secoiridoid glycoside, which is stably kept in a compartment separate from activating enzymes. When the leaf tissue is destroyed by herbivores, enz...
Chikara Hirayama, Hitoshi Saito, Kotaro Konno, Hiroshi Shinbo
Insect Biochemistry and Molecular Biology   28 473-482   Jul 1998
NADH-dependent glutamate synthase (NADH-GOGAT; EC was purified 766-fold from the fat body of 5th instar larvae of the silkworm with a final specific activity of 13.8 units/mg protein by a procedure including ammonium sulfate fraction, Q-...
Kotaro Konno, Hiroe Yasui, Chikara Hirayama, Hiroshi Shinbo
Journal of Chemical Ecology   24 735-751   Jan 1998
In previous studies, we reported the existence of a high concentration of free glycine in the digestive juice of several Lepidoptera larvae, particularly in the digestive juice of species that feed on the privet tree, Ligustrum obtusifolium. The w...
Chikara Hirayama, Kotaro Konno, Hiroshi Shinbo
Journal of Insect Physiology   43 959-964   Oct 1997
Ammonia can easily be assimilated into amino acids and used for silk-protein synthesis in the silkworm, Bombyx mori. To determine the metabolic pathway of ammonia assimilation, silkworm larvae were injected with methionine sulfoximine (MS), a spec...
K. Konno, C. Hirayama, H. Shinbo
Journal of Insect Physiology   43 217-224   Aug 1997
A large amount of free glycine was found in the digestive juice of several Lepidoptera larvae and appears to be secreted. The amount of glycine differs greatly among species and seems to correlate With the host plant. Several species of Lepidopter...
Kotaro Konno, Kotaro Konno, Chikara Hirayama, Hiroshi Shinbo
Comparative Biochemistry and Physiology - A Physiology   115 229-235   Nov 1996
In the course of investigating the digestion and the absorption of dietary protein by the silkworm, Bombyx mori, reared on an artificial diet, we found the existence of an unusually high concentration of free glycine in the midgut content compared...
Hiroshi Shinbo, Kotaro Konno, Chikara Hirayama, Kijiro Watanabe
Journal of Insect Physiology   42 1129-1138   Nov 1996
The present study was conducted to identify where dietary proteins are digested and where the digested amino acids are absorbed in the midgut of the silkworm, Bombyx mori. Analysis by electrophoresis on SDS-polyacrylamide gels shows that few of th...
Chikara Hirayama, Kotaro Konno, Hiroshi Shinbo
Journal of Insect Physiology   42 983-988   Sep 1996
This study evaluated the relative effectiveness of ammonium salt in comparison to non-essential amino acids for growth of the silkworm larvae, Bombyx mori. Newly ecdysed 5th-instar larvae were fed chemically defined diets containing only the ten e...