To evaluate the influence of sugars (glucose, fructose, lactose) and aspartame on the uptake and transport of iron, we investigated iron uptake by monolayers of Caco-2 cells and the transport of iron from the apical to the basal chamber when Caco-2 cells were grown on polycarbonate membrane inserts in serum-free medium (ITS) (ITS; insulin, transferrin and selenious acid). Iron uptake in fructose and glucose was significantly greater than in lactose and the control groups (p<0.05). When the concentration of added fructose or glucose was increased from 2 μM to 20 μM, the uptake and transport of iron significantly increased. Aspartame 2 μM increased the iron uptake and transport rate.