2012年5月
Protease-Activated Receptor-Stimulated Interleukin-6 Expression in Endometriosis-Like Lesions in an Experimental Mouse Model of Endometriosis
JOURNAL OF PHARMACOLOGICAL SCIENCES
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- 巻
- 119
- 号
- 1
- 開始ページ
- 40
- 終了ページ
- 51
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1254/jphs.11216FP
- 出版者・発行元
- JAPANESE PHARMACOLOGICAL SOC
The present study was undertaken to investigate the function of protease-activated receptor (PAR) in endometriotic lesions using a mouse model of endometriosis. Unilateral ovariectomy (uOVX) was performed on female nude mice followed by intraperitoneal transplantation of a suspension mixture of immortalized human endometrial epithelial cells (EM-1) and stromal cells (EtsT-499). Endometriosis-like lesions were observed mostly around the dissection site after transplantation. The expression of interleukin (IL)-6 and cyclooxygenase-2 in the lesions was enhanced in uOVX mice compared to non-uOVX animals. In non-uOVX mice, IL-6 mRNA levels were higher in lesions formed with cells that were pretreated with PAR1/2 agonists (thrombin, 10 U/ml and PAR2-activating peptide, 30 mu M) compared to untreated, intact cells. Peritoneal IL-6 concentrations were also increased in the PAR1/2 agonists treated group. IL-6 expression induced by PAR activation was blocked by the treatment of cells with serine protease inhibitors. In cultured endometrial cells, simultaneous treatment with PAR1 and PAR2 agonists significantly increased the expression of IL-6. These results suggest that peritoneal bleeding may accelerate IL-6 expression in endometriotic lesions in part through the activation of PAR.
- リンク情報
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- DOI
- https://doi.org/10.1254/jphs.11216FP
- CiNii Articles
- http://ci.nii.ac.jp/naid/40019255670
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/22641126
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000304490300005&DestApp=WOS_CPL
- ID情報
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- DOI : 10.1254/jphs.11216FP
- ISSN : 1347-8613
- CiNii Articles ID : 40019255670
- PubMed ID : 22641126
- Web of Science ID : WOS:000304490300005