論文

査読有り 国際誌
2020年11月26日

Prosaposin in the rat oviductal epithelial cells.

Cell and tissue research
  • Tetsuya Shimokawa
  • ,
  • Hiroaki Nabeka
  • ,
  • Sakirul Islam Khan
  • ,
  • Kimiko Yamamiya
  • ,
  • Takuya Doihara
  • ,
  • Naoto Kobayashi
  • ,
  • Hiroyuki Wakisaka
  • ,
  • Seiji Matsuda

記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00441-020-03339-x

Prosaposin (PSAP) has two forms: a precursor and a secreted form. The secreted form has neurotrophic, myelinotrophic, and myotrophic properties. The precursor form is a precursor protein of saposins A-D. Although the distribution of PSAP in male reproductive organs is well known, its distribution in female reproductive organs, especially in the oviduct, is unclear. Immunoblots and immunohistochemistry of oviducts showed that oviductal tissues contain PSAP proteins, and a significant increase in PSAP was observed in the estrus-metestrus phase compared to the diestrus-proestrus phase in the ampulla. To identify PSAP trafficking in cells, double-immunostaining was performed with antibodies against PSAP in combination with sortilin, mannose 6 phosphate receptor (M6PR), or low-density lipoprotein receptor-related protein 1 (LRP1). PSAP and sortilin double-positive reactions were observed near the nuclei, as well as in the apical portion of microvillous epithelial cells, whereas these reactions were only observed near the nuclei of ciliated epithelial cells. PSAP and M6PR double-positive reactions were observed near the nuclei of microvillous and ciliated epithelial cells. PSAP and M6PR double-positive reactions were also observed in the apical portion of microvillous epithelial cells. PSAP and LRP1 double-positive reactions were observed in the plasma membrane and apical portion of both microvillous and ciliated epithelial cells. Immunoelectron staining revealed PSAP immunoreactive small vesicles with exocytotic features at the apical portion of microvillous epithelial cells. These findings suggest that PSAP is present in the oviductal epithelium and has a pivotal role during pregnancy in providing an optimal environment for gametes and/or sperm in the ampulla.

リンク情報
DOI
https://doi.org/10.1007/s00441-020-03339-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/33242172

エクスポート
BibTeX RIS