2017年11月
Quantitative analysis of O-GlcNAcylation in combination with isobaric tag labeling and chemoenzymatic enrichment
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
- ,
- ,
- ,
- 巻
- 27
- 号
- 22
- 開始ページ
- 5022
- 終了ページ
- 5026
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1016/j.bmcl.2017.10.005
- 出版者・発行元
- PERGAMON-ELSEVIER SCIENCE LTD
Protein O-GlcNAcylation regulates various biological processes, and is associated with several diseases. Therefore, the development of quantitative proteomics is important for understanding the mechanisms of O-GlcNAc-related diseases. We previously reported selective enrichment of O-GlcNAcylated peptides, which provided high-selectivity and effective release by a novel thiol-alkyne and thiol-disulfide exchange. Here, we describe a new approach using initial isobaric tag labeling for relative quantification followed by enrichment and beta-elimination/Michael addition with dithiothreitol for identification of both proteins and modification sites. The approach was validated using model proteins and peptides. This novel strategy could be used for quantitative O-GlcNAcome of biological samples. (C) 2017 Elsevier Ltd. All rights reserved.
- リンク情報
- ID情報
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- DOI : 10.1016/j.bmcl.2017.10.005
- ISSN : 0960-894X
- eISSN : 1464-3405
- PubMed ID : 29029932
- Web of Science ID : WOS:000414000000017