Tissue regeneration is the important issue in the field of operative dentistry. In the previous study, we demonstrated that TNFα activates the potential for totipotency in human bone marrow mesenchymal stem cells (hMSCs) and leads to the regeneration of target cells or tissues along with the appropriate differentiating factor such as Emd-Gel. In this study, we apply the transcription factor set of TNF-α and Amelogenin to the human adipose-derived stem cells (hASCs) and our results indicate that this transcription factor set differentiate hASCs into odontblasts simillary and suggest that TNFα activates potential totipotency of hASCs and the activation can lead to the regeneration of target cells or tissues