1996年1月
Effects of subtle changes in the SU protein of ecotropic murine leukemia virus on its brain capillary endothelial cell tropism and interference properties
VIROLOGY
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- 巻
- 215
- 号
- 2
- 開始ページ
- 142
- 終了ページ
- 151
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- 出版者・発行元
- ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
PVC-211 murine leukemia virus (MuLV) is a neuropathogenic variant of Friend MuLV (F-MuLV) that causes a rapidly progressive neurodegenerative disease in susceptible rodents. PVC-211 MuLV, but not the parental F-MuLV, can infect rat brain capillary endothelial cells (BCEC) efficiently, and the major determinant for BCEC tropism of PVC-211 MuLV is localized within the Xbal-BamHI fragment of the viral genome containing the 5' half of the env gene. To further dissect the Xbal-BamHI region for its effects on BCEC tropism, we constructed recombinant viruses between PVC-211 MuLV and F-MuLV and tested their infectivity on a cell line established from rat BCEC. Our results indicated that Glu(116)-to-Gly and Glu(129)-to-Lys substitutions in the background of the F-MuLV envelope SU protein were sufficient for conferring BCEC tropism on the virus. Interference studies of these viruses on Rat-1 fibroblastic cells showed that the structure of the SU protein encoded by the Xbal-BamHI region also has significant effects on their affinity for the rat ecotropic MuLV receptor. These results support the possibility that structural elements I and II of the SU protein are important determinants for virus-receptor interaction. (C) 1996 Academic Press, Inc.
- リンク情報
- ID情報
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- ISSN : 0042-6822
- Web of Science ID : WOS:A1996TQ89300004