論文

査読有り
2018年3月

Analysis of major paralogs encoding the Fra a 1 allergen based on their organ-specificity in Fragaria x ananassa

PLANT CELL REPORTS
  • Ishibashi, Misaki
  • ,
  • Nabe, Takeshi
  • ,
  • Nitta, Yoko
  • ,
  • Tsuruta, Hiroki
  • ,
  • Iduhara, Miho
  • ,
  • Uno, Yuichi

37
3
開始ページ
411
終了ページ
424
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1007/s00299-017-2237-6
出版者・発行元
SPRINGER

Fra a 1 protein in strawberry causes oral allergic syndrome. Over 39 Fra a 1 paralogs have been identified in strawberry genome. Fra a 1.01 is major accumulating protein in edible organs.Strawberry fruits contain allergenic proteins that cause oral allergic syndrome. The hypothesized major allergen is Fra a 1, an ortholog of the birch pollen allergen protein Bet v 1. We organized Fra a 1 genes and analyzed their localizations at the transcriptional and translational levels. In total, 15 new Fra a 1 proteins were identified from the genomic database, increasing the total number of Fra a 1 to 30 proteins encoded by 39 genes. Fra a 1.02 was mostly expressed in receptacles, and Fra a 1.01 in achenes, when analyzed by RNA sequencing. Immunoblotting showed that the Fra a 1.01 protein was broadly accumulated in strawberry organs, while the Fra a 1.02 protein was mostly expressed in receptacles. Recombinant Fra a 1.01 strongly reacted with human IgE. The mRNA and protein expression levels of Fra a 1 did not correlate, indicating the importance of protein levels when evaluating the abundance of allergens in strawberry. Based on the localizations, accumulation levels and reactivity to human IgE, we determined that Fra a 1.01 was the most important allergen, followed by Fra a 1.02, and then other Fra a 1 proteins. The information obtained here will be useful for selecting the target Fra a 1 paralogs when breeding hypoallergenic strawberry.

Web of Science ® 被引用回数 : 10

リンク情報
DOI
https://doi.org/10.1007/s00299-017-2237-6
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000426064000003&DestApp=WOS_CPL
ID情報
  • DOI : 10.1007/s00299-017-2237-6
  • ISSN : 0721-7714
  • eISSN : 1432-203X
  • Web of Science ID : WOS:000426064000003

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