論文

査読有り
2017年6月

Expression Profiling of Strawberry Allergen Fra a during Fruit Ripening Controlled by Exogenous Auxin

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
  • Ishibashi, Misaki
  • ,
  • Yoshikawa, Hiroki
  • ,
  • Uno, Yuichi

18
6
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/ijms18061186
出版者・発行元
MDPI

Strawberry fruit contain the allergenic Fra a proteins, members of the pathogenesis-related 10 protein family that causes oral allergic syndrome symptoms. Fra a proteins are involved in the flavonoid biosynthesis pathway, which might be important for color development in fruits. Auxin is an important plant hormone in strawberry fruit that controls fruit fleshiness and ripening. In this study, we treated strawberry fruits with exogenous auxin or auxin inhibitors at pre- and post-harvest stages, and analyzed Fra a transcriptional and translational expression levels during fruit development by real-time PCR and immunoblotting. Pre-harvest treatment with 1-naphthaleneacetic acid (NAA) alone did not affect Fra a expression, but applied in conjunction with achene removal NAA promoted fruit pigmentation and Fra a protein accumulation. The response was developmental stage-specific: Fra a 1 was highly expressed in immature fruit, whereas Fra a 2 was expressed in young to ripe fruit. In post-harvest treatments, auxin did not contribute to Fra a induction. Auxin inhibitors delayed fruit ripening; as a result, they seemed to influence Fra a 1 expression. Thus, Fra a expression was not directly regulated by auxin, but might be associated with the ripening process and/or external factors in a paralog-specific manner.

Web of Science ® 被引用回数 : 7

リンク情報
DOI
https://doi.org/10.3390/ijms18061186
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000404581500085&DestApp=WOS_CPL
ID情報
  • DOI : 10.3390/ijms18061186
  • eISSN : 1422-0067
  • Web of Science ID : WOS:000404581500085

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