論文

査読有り
2021年

Enzymatic Activities and Gene Transcript Levels Associated with the Augmentation of Antioxidant Constituents during Drought Stress in Lettuce

Horticulturae
  • Ryohei Koyama
  • ,
  • Aika Yoshimoto
  • ,
  • Misaki Ishibashi
  • ,
  • Hiromichi Itoh
  • ,
  • Yuichi Uno

7
11
開始ページ
444
終了ページ
444
記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.3390/horticulturae7110444
出版者・発行元
MDPI AG

Efficient cultivation methods were investigated to promote the branding of products in plant factories. Moderate stress can enhance plant constituents that are beneficial for human health, without reducing yield. Dehydration stress in lettuce rhizospheres increased some antioxidants, including L-ascorbic acid (AsA) and polyphenols. In this study, the major factors contributing to the augmentation of antioxidant constituents were investigated. The drought treatment resulted in increased hydrophilic oxygen radical absorbance capacity (ORAC) values but not hydrophobic ORAC values. Both activities of antioxidant enzymes (superoxide dismutase, SOD, and ascorbate peroxidase, APX) were elevated under drought conditions. RNA-seq analysis revealed 33 upregulated and 115 downregulated differentially expressed genes, and 40 gene ontology enrichment categories. A dehydrin gene was the most significant among the upregulated genes in response to drought stress. Dehydrin protects plant cells from dryness through multiple functions, such as radical scavenging and protection of enzymes. Real-time PCR validated the substantial increase in some dehydrin paralogs with root desiccation. In conclusion, the enhancement of antioxidant levels by drought stress is likely not due to the induction of antioxidant enzyme genes, but due to increased enzymic activities. These activities might be protected by dehydrins encoded by the upregulated paralogs under drought stress.

リンク情報
DOI
https://doi.org/10.3390/horticulturae7110444
URL
https://www.mdpi.com/2311-7524/7/11/444/pdf
ID情報
  • DOI : 10.3390/horticulturae7110444
  • eISSN : 2311-7524

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