2013年
Effects of Interleukin-31 on MUC5AC Gene Expression in Nasal Allergic Inflammation
PHARMACOLOGY
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- 巻
- 91
- 号
- 3-4
- 開始ページ
- 158
- 終了ページ
- 164
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1159/000346609
- 出版者・発行元
- KARGER
Background: Interleukin-31 (IL-31) is a newly discovered T helper lymphocyte-derived cytokine that plays an important role in allergic inflammation. However, the effects of IL-31 on mucus production in nasal allergic inflammation are completely unknown. Objective: To investigate the effects of IL-31 on mucin gene expression (MUC5AC) in patients with allergic rhinitis and in human airway epithelial cells. Methods: Expression levels of IL-31 and IL-31 receptor A (IL-31 RA) were evaluated in the inferior turbinate of patients with allergic rhinitis and non-allergic rhinitis with innmunohistochemistry. IL-31-induced MUC5AC gene expression was measured with a MUC5AC luciferase reporter assay in human epithelial HM3-MUC5AC cells and quantified by quantitative real-time polymerase chain reaction in human airway epithelial A549 cells. Results: IL-31RA was primarily localized in submucosal glands and upregulated in allergic rhinitis. IL-31 was detected in submucosal tissue and increased in allergic inflammation. MUC5AC gene expression was induced by IL-31 stimulation both in HM3-MUC5AC and A549 cells. Additionally, IL-31 cooperated with Th2 cytokines on MUC5AC gene expression in HM3-MUC5AC cells. Conclusion: IL-31 and IL-31 RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. These findings suggest that IL-31 plays an important role in mucus overproduction in nasal allergic inflammation. Copyright (C) 2013 S. Karger AG, Basel
- リンク情報
- ID情報
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- DOI : 10.1159/000346609
- ISSN : 0031-7012
- PubMed ID : 23392388
- Web of Science ID : WOS:000319086500006