論文

査読有り
2013年

Effects of Interleukin-31 on MUC5AC Gene Expression in Nasal Allergic Inflammation

PHARMACOLOGY
  • Said Ahmad Shah
  • ,
  • Hajime Ishinaga
  • ,
  • Bo Hou
  • ,
  • Mitsuhiro Okano
  • ,
  • Kazuhiko Takeuchi

91
3-4
開始ページ
158
終了ページ
164
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1159/000346609
出版者・発行元
KARGER

Background: Interleukin-31 (IL-31) is a newly discovered T helper lymphocyte-derived cytokine that plays an important role in allergic inflammation. However, the effects of IL-31 on mucus production in nasal allergic inflammation are completely unknown. Objective: To investigate the effects of IL-31 on mucin gene expression (MUC5AC) in patients with allergic rhinitis and in human airway epithelial cells. Methods: Expression levels of IL-31 and IL-31 receptor A (IL-31 RA) were evaluated in the inferior turbinate of patients with allergic rhinitis and non-allergic rhinitis with innmunohistochemistry. IL-31-induced MUC5AC gene expression was measured with a MUC5AC luciferase reporter assay in human epithelial HM3-MUC5AC cells and quantified by quantitative real-time polymerase chain reaction in human airway epithelial A549 cells. Results: IL-31RA was primarily localized in submucosal glands and upregulated in allergic rhinitis. IL-31 was detected in submucosal tissue and increased in allergic inflammation. MUC5AC gene expression was induced by IL-31 stimulation both in HM3-MUC5AC and A549 cells. Additionally, IL-31 cooperated with Th2 cytokines on MUC5AC gene expression in HM3-MUC5AC cells. Conclusion: IL-31 and IL-31 RA are upregulated in patients with allergic rhinitis, and induce MUC5AC gene expression in human airway epithelial cells. These findings suggest that IL-31 plays an important role in mucus overproduction in nasal allergic inflammation. Copyright (C) 2013 S. Karger AG, Basel

リンク情報
DOI
https://doi.org/10.1159/000346609
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/23392388
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000319086500006&DestApp=WOS_CPL
ID情報
  • DOI : 10.1159/000346609
  • ISSN : 0031-7012
  • PubMed ID : 23392388
  • Web of Science ID : WOS:000319086500006

エクスポート
BibTeX RIS