Although dilution of lake water has been used for improvement of water quality and algal blooms control, it has not necessarily succeeded to suppress the blooms. We hypothesized that the disappearance of algal blooms by dilution could be explained by flow regime, nutrient concentrations, and their interaction. This study investigated the effects of daily renewal rate (d), nitrogen (N) and phosphorus (P) concentration, and their interaction on the domination between Microcystis aeruginosa and Cyclotella sp. through a monoxenic culture experiment. The simulation model as functions of the N: P mass ratio and dilution rate (D) (calculated from d) was constructed, and the dominant characteristics of both species were predicted based on the model using parameters obtained in a monoculture experiment and our previous study. Results of monoxenic culture experiment revealed that M. aeruginosa dominated in all conditions (d = 5 or 15%; N = 1.0 or 2.5 or 5.0 mg-N L-1; P = 0.1 or 0.5 mg-P L-1) and the predicted cell densities were substantially correspondent to experimental data. Under various N: P ratios and D values, characteristics of domination for each species were predicted, indicating that Cyclotella sp. tended to be dominant under high P concentrations (P = 0.36 mg-P L-1) when the N: P ratio was less than 7.0, and M. aeruginosa could not form algal blooms at the N: P ratio = 7.0 (N = 0.7 mg-N L-1). It was also suggested that the dilution rate leading to the Cyclotella sp. domination required 0.20 day(-1) or higher regardless of the N: P ratios.