2017年1月
Biological activity of peptide-conjugated polyion complex matrices consisting of alginate and chitosan
BIOPOLYMERS
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- 巻
- 108
- 号
- 1
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1002/bip.22983
- 出版者・発行元
- WILEY
Peptide-conjugated polysaccharide matrices using bioactive laminin-derived peptides are useful biomaterials for tissue and cell engineering. Here, we demonstrate an easy handling preparation method for peptide-polysaccharide matrices using polyion complex with both alginate and chitosan. First, aldehyde-alginate was synthesized by oxidization of alginate using NaIO4, and then, reacted with Cys-peptides. Next, the peptide-alginate solution was added to a chitosan-coated plate, and the peptide-polyion complex matrices (peptide-PCMs) were prepared. The peptide-PCMs using an integrin alpha v beta 3-binding peptide (A99a: ALRGDN, mouse laminin alpha 1 chain 1145-1150) and an integrin alpha v beta 1-binding peptide (EF1XmR: RLQLQEGRLHFXFD, X=Nle, mouse laminin alpha 1 chain 2751-2763) showed strong cell attachment activity in a dose-dependent manner. When we examined the effect of various spacers on the biological activity of A99a-PCM, hydrophobic and long spacers enhanced the cell attachment activity. Further, the A99a-PCM with the spacers strongly promoted neurite outgrowth. The polyion complex method is an easy way to obtain insolubilized matrix and is widely applicable for various polysaccharides. The peptide-PCM is useful as a biomaterial for cell and tissue engineering.
- リンク情報
- ID情報
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- DOI : 10.1002/bip.22983
- ISSN : 0006-3525
- eISSN : 1097-0282
- Web of Science ID : WOS:000407991400006