論文

2022年12月

Dried human cultured epidermis accelerates wound healing in diabetic mouse skin defect wounds

Scientific Reports
  • Michiharu Sakamoto
  • ,
  • Takashi Nakano
  • ,
  • Itaru Tsuge
  • ,
  • Hiroki Yamanaka
  • ,
  • Yasuhiro Katayama
  • ,
  • Yoshihiro Shimizu
  • ,
  • Yoshika Note
  • ,
  • Masukazu Inoie
  • ,
  • Naoki Morimoto

12
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記述言語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41598-022-07156-w
出版者・発行元
Springer Science and Business Media LLC

<title>Abstract</title>Cryopreserved allogeneic cultured epidermis (CE) is used for treating second-degree burn wounds and diabetic foot ulcers; however, the need for cryopreservation limits its use. We have previously reported that CE accelerates wound healing irrespective of its viability and hypothesized that dehydrated CEs lacking living cells may act as an effective wound dressing. We prepared dried CE and investigated its morphological and physical properties and wound-healing effects and compared them with those of cryopreserved CE. Hematoxylin–eosin staining, immunostaining for basement membrane, and electron microscopy revealed that the morphologies of dried CE and cryopreserved CE were comparable and that the membrane structure was not damaged. The breaking strength, modulus of elasticity, and water permeability of dried CE were comparable with those of the cryopreserved CE. Furthermore, the levels of various active cytokines and chemokines in dried CE were comparable with those in cryopreserved CE. Dried CE applied to skin defect in diabetic mice significantly reduced the wound area and increased the new epithelium length 4 and 7 days after implantation, similar to that observed for cryopreserved CE. Consequently, dried CE had similar morphological and physical properties and wound-healing effects compared with those of cryopreserved CE and can be a physiological and versatile wound-dressing.

リンク情報
DOI
https://doi.org/10.1038/s41598-022-07156-w
URL
https://www.nature.com/articles/s41598-022-07156-w.pdf
URL
https://www.nature.com/articles/s41598-022-07156-w
ID情報
  • DOI : 10.1038/s41598-022-07156-w
  • eISSN : 2045-2322

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