論文

査読有り
2012年3月

A tonoplast-localized half-size ABC transporter is required for internal detoxification of aluminum in rice

Plant Journal
  • Chao-Feng Huang
  • ,
  • Naoki Yamaji
  • ,
  • Zhichang Chen
  • ,
  • Jian Feng Ma

69
5
開始ページ
857
終了ページ
867
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1111/j.1365-313X.2011.04837.x

Toxic aluminum enters the root cells rapidly, therefore internal detoxification is required. However, the molecular mechanisms underlying this process are poorly understood. Here we functionally characterized a rice gene, Os03g0755100 (OsALS1), that is regulated by ART1, a C2H2-type zinc finger transcription factor. OsALS1 encodes a half-size ABC transporter that is a member of the TAP (transporter associated with antigen processing) sub-group. Expression of OsALS1 was rapidly and specifically induced by Al in the roots, but not by other metals or low pH. OsALS1 was localized at all cells of the roots. Furthermore, OsALS1 is localized to the tonoplast. These expression patterns and cell specificity of localization are different from those of the homologous gene AtALS1 in Arabidopsis. Knockout of OsALS1 in three independent lines resulted in significant increased sensitivity to Al, but did not affect the sensitivity to other metals and low pH. Comparison of Al accumulation patterns between wild-type and osals1 mutants showed that there was no difference in Al levels in the cell sap of root tips between wild-type and the mutants, but the mutants accumulated more Al in the cytosol and nucleus than the wild-type. Expression of OsALS1 in yeast resulted in increased Al sensitivity due to mis-localization. These results indicate that OsALS1 localized at the tonoplast is responsible for sequestration of Al into the vacuoles, which is required for internal detoxification of Al in rice. © 2011 Blackwell Publishing Ltd.

リンク情報
DOI
https://doi.org/10.1111/j.1365-313X.2011.04837.x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/22035218
URL
http://orcid.org/0000-0002-7499-3004
Scopus
https://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84857640657&origin=inward 本文へのリンクあり
Scopus Citedby
https://www.scopus.com/inward/citedby.uri?partnerID=HzOxMe3b&scp=84857640657&origin=inward
ID情報
  • DOI : 10.1111/j.1365-313X.2011.04837.x
  • ISSN : 0960-7412
  • ISSN : 1365-313X
  • eISSN : 1365-313X
  • ORCIDのPut Code : 9166164
  • PubMed ID : 22035218
  • SCOPUS ID : 84857640657

エクスポート
BibTeX RIS