論文

査読有り
1999年9月

Characterization of a novel kinetochore protein, CENP-H

JOURNAL OF BIOLOGICAL CHEMISTRY
  • N Sugata
  • ,
  • E Munekata
  • ,
  • K Todokoro

274
39
開始ページ
27343
終了ページ
27346
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1074/jbc.274.39.27343
出版者・発行元
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC

Macromolecular centromere-kinetochore complex plays a critical role in sister chromatid separation, but its complete protein composition as well as its precise dynamic function during mitosis has not yet been clearly determined. Here we report the isolation of a novel mouse kinetochore protein, CENP-H. The CENP-H, with an apparent molecular mass of 33 kDa, was found to contain a coiled-coil structure and a nuclear localization signal. The CENP-H transcripts were relatively scarce but were detectable in most tissues and embryos at various stages of development. Immunofluorescence stainings of mouse fibroblast cells with anti-CENP-H-specific antibody demonstrated that the CENP-H is specifically and constitutively localized in kinetochores throughout the cell cycle; this was also confirmed by stainings with anti-centromere-specific antibody. Thus the newly isolated CENP-H may play a role in kinetochore organization and function throughout the cell cycle.

Web of Science ® 被引用回数 : 64

リンク情報
DOI
https://doi.org/10.1074/jbc.274.39.27343
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/10488063
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000082739100002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1074/jbc.274.39.27343
  • ISSN : 0021-9258
  • PubMed ID : 10488063
  • Web of Science ID : WOS:000082739100002

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