2016年5月
Discovery of Potential Biomarkers with Dose- and Time-Dependence in Cisplatin-Induced Nephrotoxicity Using Metabolomics Integrated with a Principal Component-Based Area Calculation Strategy
CHEMICAL RESEARCH IN TOXICOLOGY
- ,
- ,
- ,
- ,
- ,
- ,
- 巻
- 29
- 号
- 5
- 開始ページ
- 776
- 終了ページ
- 783
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1021/acs.chemrestox.5b00519
- 出版者・発行元
- AMER CHEMICAL SOC
Cisplatin is a potent chemotherapeutic agent. However, its clinical usage is restricted by serious adverse effects, especially nephrotoxicity. For revealing the dose- and time-dependence of cisplatin-induced nephrotoxicity, mass spectrometry based metabolomics integrated with a principal component-based area calculation (PCAC) strategy was proposed in the present study. Area plots based on the first two principal components of the principal component analysis model were constructed first. Then, the sums of cumulative areas under PC-T curves (AUC(PC-T)) were calculated. Finally, the fold change of AUC(PC-T) between experimental and control groups at different time points was calculated and used as an indicative parameter. With the PCAC approach, dose- and time-dependence of cisplatin-induced metabolic change was quantitatively confirmed for the first time. Furthermore, 27 potential biomarkers with dose- and time-dependence related to nephrotoxicity induced by cisplatin were screened out and tentatively identified. Metabolic pathways interrupted by cisplatin mainly included energy, amino acid, and lipid metabolism.
- リンク情報
- ID情報
-
- DOI : 10.1021/acs.chemrestox.5b00519
- ISSN : 0893-228X
- eISSN : 1520-5010
- Web of Science ID : WOS:000376144100007