Papers

Peer-reviewed
7 2009

Expression of mRNA for 3HADH in manipulated embryos to produce germline chimeric chickens

CYTOTECHNOLOGY
  • Hiroki Furuta
  • ,
  • Yuko Yoshimi
  • ,
  • Yoshiyuki Ohta
  • ,
  • Tatsuyuki Yoshida

Volume
60
Number
1-3
First page
165
Last page
168
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1007/s10616-009-9232-x
Publisher
SPRINGER

Germline chimeric chickens were produced by the transfer of primordial germ cells (PGCs) or blastoderm cells. The hatchability of eggs produced by transfer of exogenous PGCs is usually low. The purpose of the present study was investigated to express (3-hydroxyacyl CoA dehydrogenase) 3HADH which is a limiting enzyme in the beta-oxidation of fatty acids for hatching energy. Manipulations of both donor and recipient eggshells were as follows. A window approximately 10 mm in diameter was opened at the pointed end of the eggs at stage 12-15 days incubation. Donor PGCs, taken from the blood vessels of donor embryos from fertilized eggs at the same stage of development, were injected into the blood vessels of recipient embryos. The muscles of chicks in the eggs with transferred PGCs were removed after 20 days of incubation. A cDNA was prepared from the total RNA. The expression of 3HADH in the manipulated embryos was investigated using real-time PCR analysis. Real-time PCR analysis showed that expression of 3HADH was reduced in the muscles of manipulated embryos.

Web of Science ® Times Cited Count : 1
View Related Records® in Web of Science ®

Link information
DOI
https://doi.org/10.1007/s10616-009-9232-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/19856125
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000272027900019&DestApp=WOS_CPL

Export
BibTeX RIS