2009年7月
Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification
JOURNAL OF CELL SCIENCE
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- 巻
- 122
- 号
- 14
- 開始ページ
- 2504
- 終了ページ
- 2513
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1242/jcs.050443
- 出版者・発行元
- COMPANY OF BIOLOGISTS LTD
The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, the origin of phagosomal V-ATPase remains poorly understood. We found that phagosomes were enriched with the V-ATPase a3 subunit, which also accumulated in late endosomes and lysosomes. We modified the mouse Tcirg1 locus encoding subunit a3, to express an a3-GFP fusion protein. Live-cell imaging and immunofluorescence microscopy revealed that nascent phagosomes received the a3-GFP from tubular structures extending from lysosomes located in the perinuclear region. Macrophages from a3-deficient mice exhibited impaired acidification of phagosomes and delayed digestion of bacteria. These results show that lysosomal V-ATPase is recruited directly to the phagosomes via tubular lysosomes to establish the acidic environment hostile to pathogens.
- リンク情報
- ID情報
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- DOI : 10.1242/jcs.050443
- ISSN : 0021-9533
- PubMed ID : 19549681
- Web of Science ID : WOS:000268257600020