論文

査読有り 国際誌
2015年9月8日

Comprehensive DNA Methylation Analysis of Retrotransposons in Male Germ Cells.

Cell reports
  • Ippei Nagamori
  • ,
  • Hisato Kobayashi
  • ,
  • Yusuke Shiromoto
  • ,
  • Toru Nishimura
  • ,
  • Satomi Kuramochi-Miyagawa
  • ,
  • Tomohiro Kono
  • ,
  • Toru Nakano

12
10
開始ページ
1541
終了ページ
7
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1016/j.celrep.2015.07.060

De novo DNA methylation of retrotransposons is critical for silencing. Here, we use DNA methylation analysis to examine retrotransposons in mouse male germ cells. DNA methylation of long interspersed nuclear elements (LINEs) is dependent on piRNA, and younger LINEs exhibit greater piRNA dependence. In contrast, most long terminal repeat (LTR) retrotransposons produce lower levels of piRNAs and do not show significant piRNA dependence. The relationship between DNA methylation and corresponding piRNA expression of several LTR retrotransposons was reduced in Mili-null cells, but not Miwi2-null cells. These observations raise the possibility of piRNA-dependent DNA methylation without Miwi2. Therefore, it appears that the molecular mechanisms of the gene silencing of retrotransposons are more complicated than previously thought.

リンク情報
DOI
https://doi.org/10.1016/j.celrep.2015.07.060
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/26321633
ID情報
  • DOI : 10.1016/j.celrep.2015.07.060
  • PubMed ID : 26321633

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