論文

査読有り 筆頭著者
2014年11月

Different Sets of ER-Resident J-Proteins Regulate Distinct Polar Nuclear-Membrane Fusion Events in Arabidopsis thaliana

PLANT AND CELL PHYSIOLOGY
  • Daisuke Maruyama
  • ,
  • Masaya Yamamoto
  • ,
  • Toshiya Endo
  • ,
  • Shuh-ichi Nishikawa

55
11
開始ページ
1937
終了ページ
1944
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1093/pcp/pcu120
出版者・発行元
OXFORD UNIV PRESS

Angiosperm female gametophytes contain a central cell with two polar nuclei. In many species, including Arabidopsis thaliana, the polar nuclei fuse during female gametogenesis. We previously showed that BiP, an Hsp70 in the endoplasmic reticulum (ER), was essential for membrane fusion during female gametogenesis. Hsp70 function requires partner proteins for full activity. J-domain containing proteins (J-proteins) are the major Hsp70 functional partners. A. thaliana ER contains three soluble J-proteins, AtERdj3A, AtERdj3B, and AtP58(IPK). Here, we analyzed mutants of these proteins and determined that double-mutant ovules lacking AtP58(IPK) and AtERdj3A or AtERdj3B were defective in polar nuclear fusion. Electron microscopy analysis identified that polar nuclei were in close contact, but no membrane fusion occurred in mutant ovules lacking AtP58(IPK) and AtERdj3A. The polar nuclear outer membrane appeared to be connected via the ER remaining at the inner unfused membrane in mutant ovules lacking AtP58(IPK) and AtERdj3B. These results indicate that ER-resident J-proteins, AtP58(IPK)/AtERdj3A and AtP58(IPK)/AtERdj3B, function at distinct steps of polar nuclear-membrane fusion. Similar to the bip1 bip2 double mutant female gametophytes, the aterdj3a atp58(ipk) double mutant female gametophytes defective in fusion of the outer polar nuclear membrane displayed aberrant endosperm proliferation after fertilization with wild-type pollen. However, endosperm proliferated normally after fertilization of the aterdj3b atp58(ipk) double mutant female gametophytes defective in fusion of the inner membrane. Our results indicate that the polar nuclear fusion defect itself does not cause an endosperm proliferation defect.

リンク情報
DOI
https://doi.org/10.1093/pcp/pcu120
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/25231968
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000346797600009&DestApp=WOS_CPL
ID情報
  • DOI : 10.1093/pcp/pcu120
  • ISSN : 0032-0781
  • eISSN : 1471-9053
  • PubMed ID : 25231968
  • Web of Science ID : WOS:000346797600009

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