2022年6月17日
Real-Time Scanning Electron Microscopy of Unfixed Tissue in Solution using a Deformable and Electron-Transmissive Film
Microscopy
- ,
- ,
- 巻
- 71
- 号
- 5
- 開始ページ
- 297
- 終了ページ
- 301
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.1093/jmicro/dfac030
- 出版者・発行元
- OXFORD UNIV PRESS
It is difficult to use scanning electron microscopy to observe the structure and movement of biological tissue immersed in solution. To enable such observations, we created a highly deformable and electron-transmissive polyimide film that can withstand the pressure difference between the high-vacuum electron column and atmospheric-pressure sample chamber. With this film, we used scanning electron microscopy to measure the intrinsic fine structure and movement of the contractile fibers of excised mouse heart immersed in physiological solution. Our measurements revealed that the excised heart is a dynamic tissue that undergoes relaxation oscillation based on a three-dimensional force balance.
- リンク情報
-
- DOI
- https://doi.org/10.1093/jmicro/dfac030
- PubMed
- https://www.ncbi.nlm.nih.gov/pubmed/35711152
- PubMed Central
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9535786
- Web of Science
- https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000820024800001&DestApp=WOS_CPL
- ID情報
-
- DOI : 10.1093/jmicro/dfac030
- ISSN : 2050-5698
- eISSN : 2050-5701
- ORCIDのPut Code : 114568920
- PubMed ID : 35711152
- PubMed Central 記事ID : PMC9535786
- Web of Science ID : WOS:000820024800001