Shigeki Arai

J-GLOBAL         Last updated: May 31, 2019 at 15:05
Shigeki Arai

Research Areas


Published Papers

Extended Structure of Pleiotropic DNA Repair-Promoting Protein PprA from the Extreme Radiation Resistance of Deinococcus radiodurans
Motoyasu Adachi, Rumi Shimizu, Chie Shibazaki, Katsuya Sato, Satoru Fujiwara, Shigeki Arai, Kazunari Narumi, Ryota Kuroki
The FASEB Journal   33 3647-3658   Mar 2019   [Refereed]
Observation of Protein and Lipid Membrane Structures in a Model Mimicking the Molecular Crowding Environment of Cells Using Neutron Scattering and Cell Debris
Hirai M, Ajito S, Arai S, Adachi M, Shimizu R, Wakamatsu K, Takata SI, Iwase H.
The Journal of Physical Chemistry B   123(15) 3189-3198   Apr 2019   [Refereed]
The non-glycosylated N-terminal domain of human thrombopoietin is a molten globule under native conditions
Arai S, Shibazaki C, Adachi M, Maeda Y, Tahara T, Kato T, Miyazaki H, Kuroki R.
FEBS Journal   286(9) 1717-1733   May 2019   [Refereed]
Hydration structures of the human protein kinase CK2α clarified by joint neutron and X-ray crystallography
Chie Shibazaki, Shigeki Arai, Rumi Shimizu, Morihisa Saeki, Takayoshi Kinoshita, Andreas Ostermann, Tobias E. Schrader, Yuzuru Kurosaki, Tomoko Sunami, Ryota Kuroki, Motoyasu Adachi
Journal of Molecular Biology   430 5094-5104   Dec 2018   [Refereed]
Improved substrate specificity for D-galactose of L-arabinose isomerase for industrial application
Fina Amreta Laksmi, Shigeki Arai, Hirohito Tsurumaru, Yoshitaka Nakamura, Budi Saksono, Masao Tokunaga, Matsujiro Ishibashi
BBA Proteins and Proteomics   1866 1084-1091   Nov 2018   [Refereed]
Expression and characterization of the Renilla luciferase with the cumulative mutation
Matsujiro Ishibashi, Ryo Kawanabe, Norie Amaba, Shigeki Arai, Fina Amreta Laksmi, Kenta Komori, Masao Tokunaga
Protein Expression and Purification   145 39-44   May 2018   [Refereed]
Shigehisa M, Amaba N, Arai S, Higashi C, Kawanabe R, Matsunaga A, Laksmi FA, Tokunaga M, Ishibashi M
Protein engineering, design & selection : PEDS   30 7-13   2017   [Refereed]
Arai S, Shibazaki C, Adachi M, Honjo E, Tamada T, Maeda Y, Tahara T, Kato T, Miyazaki H, Blaber M, Kuroki R
Protein science : a publication of the Protein Society   25 1786-1796   Jul 2016   [Refereed]
Yonezawa Y, Nagayama A, Tokunaga H, Ishibashi M, Arai S, Kuroki R, Watanabe K, Arakawa T, Tokunaga M
The protein journal   34 275-283   Aug 2015   [Refereed]
Arai S, Yonezawa Y, Okazaki N, Matsumoto F, Shibazaki C, Shimizu R, Yamada M, Adachi M, Tamada T, Kawamoto M, Tokunaga H, Ishibashi M, Blaber M, Kuroki R
Acta crystallographica. Section D, Biological crystallography   71 541-554   Feb 2015   [Refereed][Invited]
Arai S, Yonezawa Y, Ishibashi M, Matsumoto F, Adachi M, Tamada T, Tokunaga H, Blaber M, Tokunaga M, Kuroki R
Acta crystallographica. Section D, Biological crystallography   21 498-510   Feb 2014   [Refereed]
Shoyama Y, Tamada T, Kurihara K, Takeuchi A, Taura F, Arai S, Blaber M, Shoyama Y, Morimoto S, Kuroki R
Journal of molecular biology   423 96-105   Jul 2012   [Refereed]
Ishibashi M, Uchino M, Arai S, Kuroki R, Arakawa T, Tokunaga M
Archives of biochemistry and biophysics   525 47-52   Jun 2012   [Refereed]
Structure of HIV-1 protease in complex with potent inhibitor KNI-272 determined by high-resolution X-ray and neutron crystallography
Adachi, M., Ohhara, T., Kurihara, K., Tamada, T., Honjo, E., Okazaki, N., Arai, S., Shoyama, Y., Kimura, K., Matsumura, H., Sugiyama, S., Adachi, H., Takano, K., Mori, Y., Hidaka, K., Kimura, T., Hayashi, Y., Kiso, Y., Kuroki, R.
Proc. Natl. Acad. Sci. USA   106 4641-4646   2009   [Refereed]
Tokunaga H, Ishibashi M, Arisaka F, Arai S, Kuroki R, Arakawa T, Tokunaga M
FEBS letters   582 1049-1054   Mar 2008   [Refereed]
Niimura N, Arai S, Kurihara K, Chatake T, Tanaka I, Bau R
Cellular and molecular life sciences : CMLS   63 85-300   Feb 2006   [Refereed]
Chatake T, Tanaka I, Umino H, Arai S, Niimura N
Acta crystallographica. Section D, Biological crystallography   61 1088-1098   Jul 2005   [Refereed]
Arai S, Chatake T, Ohhara T, Kurihara K, Tanaka I, Suzuki N, Fujimoto Z, Mizuno H, Niimura N
Nucleic acids research   33 3017-3024   May 2005   [Refereed]
Arai S, Chatake T, Suzuki N, Mizuno H, Niimura N
Acta crystallographica. Section D, Biological crystallography   60 1032-1039   May 2004   [Refereed]
Formation of protein crystals (orthorhombic lysozyme) in quasi-microgravity environment obtained by superconducting magnet
Yin, D.C., Wakayama, N.I., Harata, K., Fujiwara, M., Kiyoshi, T., Wada, H., Niimura, N., Arai, S., Huang, W.D., Tanimoto, Y.
Journal of Crystal Growth   270 184-191   2004   [Refereed]
Chatake T, Mizuno N, Voordouw G, Higuchi Y, Arai S, Tanaka I, Niimura N
Acta crystallographica. Section D, Biological crystallography   59 2306-2309   Nov 2003   [Refereed]
Crystallization of a large single crystal of a B-DNA decamer for a neutron diffraction experiment by the phase diagram technique
Arai, S., Chatake, T., Minezaki, Y., Niimura, N.
Acta Crystallographica Section D   58 151-153   2002   [Refereed]
Concentration dependence of thermal structural transition of hen egg-white lysozyme under constant heating rate studied by time-resolved SAXS
Hirai, M., Arai, S., Iwase. H.
Thermochimica Acta   344 95-102   2000   [Refereed]
Arai S, Hirai M
Biophysical journal   76 2192-2197   Apr 1999   [Refereed]
Comparison of DMSO-induced denaturation of hen egg-white lysozyme and bovine a-lactalbumin
Iwase, H., Hirai, M., Arai, S., Mitsuya, S., Shimizu, S., Otomo, T., Furusaka, M.
Journal of Physics and Chemistry of Solids   60 1379-1381   1999   [Refereed]
Internal structural change of ganglioside micelle depending on temperature observed by neutron solvent contrast variation
Hirai, M., Iwase, H., Hayakawa, T., Arai, S., Mitsuya, S., Nagao, M., Matsushita, Y.
Journal of Physics and Chemistry of Solids   60 1297-1299   1999   [Refereed]
Dynamics of w/o AOT microemulsions studied by neutron spin echo
Hirai, M., Hirai, R.K., Iwase, H., Arai, S., Mitsuya, S., Takeda, T., Seto, H., Nagao, M.
Journal of Physics and Chemistry of Solids   60 1359-1361   1999   [Refereed]
Complimentary analysis of thermal transition multiplicity of hen egg-white lysozome at low pH using X-ray scattering and scanning calorimetry
Hirai, M., Arai, S., Iwase, H.
The Journal of Physical Chemistry B   103 549-556   1999   [Refereed]
Hirai M, Iwase H, Arai S, Takizawa T, Hayashi K
Biophysical journal   74 1380-1387   Mar 1998   [Refereed]
Small-angle X-ray scattering and calorimetric studies of thermal conformational change of lysozyme depending on pH
Hirai, M., Arai, S., Iwase, H., Takizawa, T.
The Journal of Physical Chemistry B   102 1308-1313   1998   [Refereed]
Characteristics of thermotropic phase transition of glycosphingolipid (ganglioside) aggregates in aqueous solution
Hirai, M., Arai, S., Takizawa, T., Yabuki, S., Nakata, Y.
Thermochimica Acta   308 93-99   1998   [Refereed]
Synchrotron radiation small-angle X-ray scattering study of structural transition and gelation of hen egg-white lysozyme depending on pH and ionic strength under isothermal heating
Arai, S., Hirai, M.
55 653-660   1998   [Refereed]
Solvent contrast variation study of w/o AOT microemulsions using small-angle neutron scattering
Hirai, R.K., Hirai, M., Iwase, H., Arai, S., Imai, M., Matsushita, Y.
Physica B   241 984-986   1997   [Refereed]
Wide-angle neutron scattering study of thermal denaturation of lysozyme
Hirai, M., Arai, S., Iwase, H., Takizawa, T., Shimizu, S., Furusaka, M.
Physica B   241 1159-1161   1997   [Refereed]
Dynamics and phase behavior of a supermacromolecular suspension under a magnetic field studied by time-resolved x-ray scattering
Hirai, M., Arai, S., Takizawa, T., Yabuki, Y., Sano, Y.
Physical Review B   55 3490-3496   1997   [Refereed]

Books etc

Development of The Cesium Absorption Technique Using Artificially Modified Protein
Shigeki Arai, Motoyasu Adachi (Part:Joint Work)
Feb 2017   

Conference Activities & Talks

Human thrombopoietin as a molten globule
Shigeki Arai
26 Jun 2018   
Discovery of Cs+ selective binding site on a halophilic protein [Invited]
Shigeki Arai
Aug 2015   
Tertiary structure and low-salt concentration adaptation mechanism of beta-lactamase from moderate halophile
Jun 2015   
Discovery of Cs selective binding site on a halophilic beta-lactamase by the anomalous X-ray diffraction analysis
Jun 2014   
X-ray crystallographic analysis of alkaline phosphatase derived from a moderate halophilic Halomonas sp.593
Shigeki Arai
Jun 2013   
Cs+ and Sr2+ recognition mechanism of halophilic β-lactamase
Shigeki Arai
Jun 2012   
X-ray crystallographic analysis of β-Lactamase derived from Chromohalobacter sp.560
Shigeki Arai
Sep 2011   
Oligomeric structure of nucleoside diphosphate kinase (HaNDK) derived from Halomonas sp. 593
Jun 2010   
Conformational change of the crystal structure of TN1-Fab fragment with and without binding of human thrombopoietin
Shigeki Arai
May 2007