論文

査読有り
2019年2月1日

Antagonistic Effect of Short Neuropeptide F on Allatotropin-Inhibited Feeding Motivation of the Silkworm Larva, Bombyx mori.

Zoological science
  • Sumihiro Matsumoto
  • ,
  • Ayako Ohara
  • ,
  • Chiaki Nagai-Okatani
  • ,
  • Yi-Jun Zhou
  • ,
  • Daiki Fujinaga
  • ,
  • Hitomi Seike
  • ,
  • Shinji Nagata

36
1
開始ページ
58
終了ページ
67
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.2108/zs180119

Here, we demonstrated an antagonistic effect of short neuropeptide F (sNPF) in modulating feeding motivation in the silkworm Bombyx mori; sNPF reduced the feeding-delaying effects caused by administration of an inhibitory peptide, allatotropin (AT). In situ hybridization and MALDI-TOF MS analysis revealed the presence of three subtypes of sNPFs (sNPF-1, -2, and -3) in the midgut enteroendocrine cells. Ca2+-imaging analyses revealed that three subtypes of sNPF receptors (sNPFRs) (BNGR-A7, -A10, and -A11) showed different affinities with the three subtypes of sNPFs. In addition, sNPF activated its signaling via ERK phosphorylation in the midgut, while mixture of sNPF and AT reduced the phosphorylation level, agreeing with the results of behavioral assay. Together, our current findings suggest that intestinal sNPF positively modulates the feeding motivation by reducing the inhibitory effects by AT within the midgut.

リンク情報
DOI
https://doi.org/10.2108/zs180119
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31116539

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