論文

査読有り 国際誌
2020年1月

Specific distribution of expression and enzymatic activity of cholesterol biosynthetic enzyme DHCR24 orthologs in the phytophagous insect.

Bioscience, biotechnology, and biochemistry
  • Haruna Fujimori
  • ,
  • Yi Jun Zhou
  • ,
  • Keisuke Fukumura
  • ,
  • Sumihiro Matsumoto
  • ,
  • Yusuke Tukamoto
  • ,
  • Shinji Nagata

84
1
開始ページ
126
終了ページ
133
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1080/09168451.2019.1667221

Insects must intake sterol compounds because of their inability to synthesize cholesterol de novo. In phytophagous insects, enzymatic conversion of phytosterols to cholesterol involving 24-dehydrocholesterol reductase (DHCR24) exerts to acquire cholesterol. Here, we reported the presence of two DHCR24 homologs in the silkworm Bombyx mori, BmDHCR24-1 and -2, with several transcript variants. Consistent with the data of spatial expression analyses by RT-PCR, predominant enzymatic activity of DHCR24 was observed in B. mori larval midgut whereas weak activity was observed in the other tissues examined. In addition, BmDHCR24-1 expression in HEK293 cells showed an enzymatic activity, but BmDHCR24-2 did not, although both BmDHCR24s were localized in the endoplasmic reticulum, where the mammalian DHCR24s are located to exert their enzymatic activities. The present data indicated that BmDHCR24-1 but not BmDHCR24-2 contributes to conversion of phytosterols to cholesterol mainly in the midgut of the phytophagous lepidopteran larvae.

リンク情報
DOI
https://doi.org/10.1080/09168451.2019.1667221
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31538545

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