論文

国際誌
2020年3月

Isolation of tissue-resident vascular endothelial stem cells from mouse liver.

Nature protocols
  • Hisamichi Naito
  • ,
  • Taku Wakabayashi
  • ,
  • Masako Ishida
  • ,
  • Chang-Hyun Gil
  • ,
  • Tomohiro Iba
  • ,
  • Fitriana Nur Rahmawati
  • ,
  • Shota Shimizu
  • ,
  • Mervin C Yoder
  • ,
  • Nobuyuki Takakura

15
3
開始ページ
1066
終了ページ
1081
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1038/s41596-019-0276-x

Endothelial cells (ECs) are fundamental components of the blood vessels that comprise the vascular system; facilitate blood flow; and regulate permeability, angiogenesis, inflammatory responses and homeostatic tissue maintenance. Accumulating evidence suggests there is EC heterogeneity in vivo. However, isolation of fresh ECs from adult mice to investigate this further is challenging. Here, we describe an easy and reproducible protocol for isolation of different types of ECs and CD157+ vascular-resident endothelial stem cells (VESCs) by mechano-enzymatic tissue digestion followed by fluorescence-activated cell sorting. The procedure was established on liver tissue but can be used to isolate ECs from other organs with minimal modification. Preparation of single-cell suspensions can be completed in 2.5 h. We also describe assays for EC clonal and network formation, as well as transcriptomic analysis of isolated ECs. The protocol enables isolation of primary ECs and VESCs that can be used for a wide range of downstream analyses in vascular research.

リンク情報
DOI
https://doi.org/10.1038/s41596-019-0276-x
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/32005982
ID情報
  • DOI : 10.1038/s41596-019-0276-x
  • PubMed ID : 32005982

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