論文

国際誌
2019年

Isolation of tissue-resident endothelial stem cells and their use in regenerative medicine.

Inflammation and regeneration
  • Tomohiro Iba
  • ,
  • Hisamichi Naito
  • ,
  • Shota Shimizu
  • ,
  • Fitriana Nur Rahmawati
  • ,
  • Taku Wakabayashi
  • ,
  • Nobuyuki Takakura

39
開始ページ
9
終了ページ
9
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1186/s41232-019-0098-9

BACKGROUND: During sprouting angiogenesis, stalk cells, localized behind tip cells, generate endothelial cells (ECs) for the elongation of new vessels. We hypothesized that stalk cells may have endothelial progenitor cell properties because of their highly proliferative ability. We conducted Hoechst dye DNA staining in ECs of preexisting blood vessels from hind limb muscle and found that endothelial-side population (E-SP) cells, which efflux Hoechst rapidly with abundant ABC transporters, show highly producing ability of ECs. We previously showed the existence of E-SP cells in hind limb muscle, retina, and liver, but not in other tissues such as adipose tissue, skin, and placenta. METHODS: We investigated the existence of E-SP cells and analyzed their proliferative ability among CD31+CD45- ECs from adipose tissue, skin, and placenta of adult mice. We also analyzed the neovascular formation of E-SP cells from adipose tissue in vivo. RESULTS: We detected E-SP cells in all tissues examined. However, by in vitro colony formation analysis on OP9 cells, we found that E-SP cells from adipose tissue and skin, but not from placenta, have highly proliferative ability. Moreover, E-SP cells from adipose tissue could contribute to the neovascular formation in hind limb ischemia model. CONCLUSION: The adipose tissue and skin are available sources to obtain endothelial stem cells for conducting therapeutic angiogenesis in regenerative medicine.

リンク情報
DOI
https://doi.org/10.1186/s41232-019-0098-9
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/31086611
PubMed Central
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6505211
ID情報
  • DOI : 10.1186/s41232-019-0098-9
  • PubMed ID : 31086611
  • PubMed Central 記事ID : PMC6505211

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