Papers

Peer-reviewed
Mar, 2008

Bombyx mori midgut membrane protein P252, which binds to Bacillus thuringiensis Cry1A, is a chlorophyllide-binding protein, and the resulting complex has antimicrobial activity

APPLIED AND ENVIRONMENTAL MICROBIOLOGY
  • Ganesh N. Pandian
  • ,
  • Toshiki Ishikawa
  • ,
  • Makoto Togashi
  • ,
  • Yasuyuki Shitomi
  • ,
  • Kohsuke Haginoya
  • ,
  • Shuhei Yamamoto
  • ,
  • Tadayuki Nishiumi
  • ,
  • Hidetaka Hori

Volume
74
Number
5
First page
1324
Last page
1331
Language
English
Publishing type
Research paper (scientific journal)
DOI
10.1128/AEM.01901-07
Publisher
AMER SOC MICROBIOLOGY

The epithelial cell membrane 252-kDa protein (P252) isolated in our laboratory from Bombyx mori midgut was shown to bind strongly with Cry1Aa, Cry1Ab, and Cry1Ac toxins of Bacillus thuringiensis (15). In the current paper, P252 was shown to bind with chlorophyllide (Chlide) to form red fluorescent protein (RFP) complex, termed Bm252RFP, with absorbance and fluorescence emission peaks at 600 nm and 620 nm, respectively. P252 at a concentration of 1 mu M is shown to bind with about 50 mu M Chlide in a positively cooperative reaction to form Bm252RFP under aerobic conditions and in the presence of light at 37 degrees C. Various parameters influencing this reaction have been optimized for efficient in vitro chemical synthesis of Bm252RFP. Circular dichroism spectra revealed that P252 is composed of a P-structure (39.8% +/- 2.2%, based on 5 samples) with negligible contribution of alpha-helix structure. When bound to Chlide, the P-structure content in the complex is reduced to 21.6% +/- 3.1% (n = 5). Since Chlide had no secondary structure, the observed reduction suggests significant conformational changes of P252 during the formation of Bm252RFP complex. Bm252RFP had antimicrobial activity against Escherichia coli, Serratia marcescens, B. thuringiensis, and Saccharomyces cerevisiae with 50% effective concentrations of 2.82, 2.94, 5.88 mu M, and 21.6 mu M, respectively. This is the first report ever to show clear, concrete binding characteristics of the midgut protein to form an RFP having significant antimicrobial activity.

Link information
DOI
https://doi.org/10.1128/AEM.01901-07
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000253792700004&DestApp=WOS_CPL
ID information
  • DOI : 10.1128/AEM.01901-07
  • ISSN : 0099-2240
  • eISSN : 1098-5336
  • Web of Science ID : WOS:000253792700004

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