2022年6月1日
Detection system for Legionella bacteria using photogate-type optical sensor
Japanese Journal of Applied Physics
- 巻
- 61
- 号
- SD
- 開始ページ
- SD1010
- 終了ページ
- SD1010
- 記述言語
- 英語
- 掲載種別
- 研究論文(学術雑誌)
- DOI
- 10.35848/1347-4065/ac5a25
- 出版者・発行元
- IOP Publishing
Abstract
This study describes a bacterial sensing system using a photogate-type fluorescence sensor for the purpose of identifying Legionella bacteria, which are known to emit different fluorescence for each bacterial species when irradiated with UV excitation light. First a weak-photocurrent measurement system in combination with a photogate-type optical sensor and a circuit board which was mounted with a current–voltage converter, an analog-to-digital converter, and a microcomputer was fabricated. Then weak-light measurement was performed by simulating the bacterial fluorescence of Legionella. A difference in the current ratio generated in the sensor was found depending on the wavelength of the LED light source, showing that the system works as a spectrometer. Finally, the system was applied to measure the fluorescence of the species of Legionella dumoffii and Legionella erythra and each species of Legionella was identified just by obtaining the current ratio.
This study describes a bacterial sensing system using a photogate-type fluorescence sensor for the purpose of identifying Legionella bacteria, which are known to emit different fluorescence for each bacterial species when irradiated with UV excitation light. First a weak-photocurrent measurement system in combination with a photogate-type optical sensor and a circuit board which was mounted with a current–voltage converter, an analog-to-digital converter, and a microcomputer was fabricated. Then weak-light measurement was performed by simulating the bacterial fluorescence of Legionella. A difference in the current ratio generated in the sensor was found depending on the wavelength of the LED light source, showing that the system works as a spectrometer. Finally, the system was applied to measure the fluorescence of the species of Legionella dumoffii and Legionella erythra and each species of Legionella was identified just by obtaining the current ratio.
- リンク情報
- ID情報
-
- DOI : 10.35848/1347-4065/ac5a25
- ISSN : 0021-4922
- eISSN : 1347-4065