論文

査読有り
2018年9月21日

Sequence-Based Characterization of Listeria monocytogenes Strains Isolated from Domestic Retail Meat in the Tokyo Metropolitan Area of Japan.

Japanese journal of infectious diseases
  • Yuko Yoshikawa
  • ,
  • Yoshitsugu Ochiai
  • ,
  • Mariko Mochizuki
  • ,
  • Takashi Takano
  • ,
  • Ryo Hondo
  • ,
  • Fukiko Ueda

71
5
開始ページ
373
終了ページ
377
記述言語
英語
掲載種別
DOI
10.7883/yoken.JJID.2017.582

The level of Listeria monocytogenes contamination of domestic retail meat in Tokyo, Japan, was assessed by comparison of isolates from 2004 to 2007 with those isolated before 2003. The overall prevalence of L. monocytogenes among these samples significantly diminished over time (1998-2003, 28.0%; 2004-2007, 17.6%) reflecting a significant decrease in the frequency of contamination of beef. Serotype 1/2a was isolated most frequently, reflecting a change in the predominant serotype in pork from 1/2c to 1/2a. We performed a simple genetic subtyping method based on 3 genes, iap, sigB, and actA, as well as traditional multilocus sequence typing to classify the allele types (ATs). No extensive variation among sequence types was detected. However, increased genetic diversity among the ATs of the 3 genes in the 2004-2007 isolates was evident. We identified AT 26 of the iap gene, which was not previously reported in Japanese isolates, and 6 ATs of the sigB gene, including 4 with nonsense mutations not currently registered in L. monocytogenes DNA databases. sigB is an evolutionally conserved gene that plays a role in the stress response. Our results indicate that the sigB gene may be relatively unstable among L. monocytogenes strains circulating in Japan.

リンク情報
DOI
https://doi.org/10.7883/yoken.JJID.2017.582
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/29848844
ID情報
  • DOI : 10.7883/yoken.JJID.2017.582
  • ISSN : 1344-6304
  • PubMed ID : 29848844

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