論文

査読有り
2004年5月

Specific detection and identification of herpes B virus by a PCR-microplate hybridization assay

JOURNAL OF CLINICAL MICROBIOLOGY
  • C Oya
  • ,
  • Y Ochiai
  • ,
  • Y Taniuchi
  • ,
  • T Takano
  • ,
  • F Ueda
  • ,
  • Y Yoshikawa
  • ,
  • R Hondo

42
5
開始ページ
1869
終了ページ
1874
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1128/jcm.42.5.1869-1874.2004
出版者・発行元
AMER SOC MICROBIOLOGY

Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.

Web of Science ® 被引用回数 : 8

リンク情報
DOI
https://doi.org/10.1128/jcm.42.5.1869-1874.2004
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/15131142
Web of Science
https://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=JSTA_CEL&SrcApp=J_Gate_JST&DestLinkType=FullRecord&KeyUT=WOS:000221424100002&DestApp=WOS_CPL
ID情報
  • DOI : 10.1128/jcm.42.5.1869-1874.2004
  • ISSN : 0095-1137
  • PubMed ID : 15131142
  • Web of Science ID : WOS:000221424100002

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