- AMER SOC MICROBIOLOGY
Herpes B virus DNA was specifically amplified by PCR, targeting the regions that did not cross-react with herpes simplex virus (HSV). The amplified products, which were shown to be highly genetic polymorphisms among herpes B virus isolates, were identified by microplate hybridization with probes generated by PCR. The products immobilized in microplate wells were hybridized with the biotin-labeled probes derived from the SMHV strain of herpes B virus. The amplified products derived from the SMHV and E2490 strains of herpes B virus were identified by microplate hybridization. PCR products amplified from the trigeminal ganglia of seropositive cynomolgus macaques were identified as herpes B virus DNA. The utility of the PCR-microplate hybridization assay for genetic detection and identification of the polymorphic region of herpes B virus was determined.
Web of Science ® 被引用回数 : 8
Web of Science ® の 関連論文(Related Records®)ビュー
- DOI : 10.1128/jcm.42.5.1869-1874.2004
- ISSN : 0095-1137
- PubMed ID : 15131142
- Web of Science ID : WOS:000221424100002