論文

国際誌
2022年11月25日

RNA-triggered protein cleavage and cell growth arrest by the type III-E CRISPR nuclease-protease.

Science (New York, N.Y.)
  • Kazuki Kato
  • Sae Okazaki
  • Cian Schmitt-Ulms
  • Kaiyi Jiang
  • Wenyuan Zhou
  • Junichiro Ishikawa
  • Yukari Isayama
  • Shungo Adachi
  • Tomohiro Nishizawa
  • Kira S Makarova
  • Eugene V Koonin
  • Omar O Abudayyeh
  • Jonathan S Gootenberg
  • Hiroshi Nishimasu
  • 全て表示

378
6622
開始ページ
882
終了ページ
889
記述言語
英語
掲載種別
研究論文(学術雑誌)
DOI
10.1126/science.add7347

The type III-E CRISPR-Cas7-11 effector binds a CRISPR RNA (crRNA) and the putative protease Csx29 and catalyzes crRNA-guided RNA cleavage. We report cryo-electron microscopy structures of the Cas7-11-crRNA-Csx29 complex with and without target RNA (tgRNA), and demonstrate that tgRNA binding induces conformational changes in Csx29. Biochemical experiments revealed tgRNA-dependent cleavage of the accessory protein Csx30 by Csx29. Reconstitution of the system in bacteria showed that Csx30 cleavage yields toxic protein fragments that cause growth arrest, which is regulated by Csx31. Csx30 binds Csx31 and the associated sigma factor RpoE (RNA polymerase, extracytoplasmic E), suggesting that Csx30-mediated RpoE inhibition modulates the cellular response to infection. We engineered the Cas7-11-Csx29-Csx30 system for programmable RNA sensing in mammalian cells. Overall, the Cas7-11-Csx29 effector is an RNA-dependent nuclease-protease.

リンク情報
DOI
https://doi.org/10.1126/science.add7347
PubMed
https://www.ncbi.nlm.nih.gov/pubmed/36423304
ID情報
  • DOI : 10.1126/science.add7347
  • PubMed ID : 36423304

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